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体内复制所需的腺病毒序列。

Adenovirus sequences required for replication in vivo.

作者信息

Wang K, Pearson G D

出版信息

Nucleic Acids Res. 1985 Jul 25;13(14):5173-87. doi: 10.1093/nar/13.14.5173.

DOI:10.1093/nar/13.14.5173
PMID:2991857
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321857/
Abstract

We have studied the in vivo replication properties of plasmids carrying deletion mutations within cloned adenovirus terminal sequences. Deletion mapping located the adenovirus DNA replication origin entirely within the first 67 bp of the adenovirus inverted terminal repeat. This region could be further subdivided into two functional domains: a minimal replication origin and an adjacent auxillary region which boosted the efficiency of replication by more than 100-fold. The minimal origin occupies the first 18 to 21 bp and includes sequences conserved between all adenovirus serotypes. The adjacent auxillary region extends past nucleotide 36 but not past nucleotide 67 and contains the binding site for nuclear factor I.

摘要

我们研究了携带克隆腺病毒末端序列内缺失突变的质粒在体内的复制特性。缺失图谱分析表明，腺病毒DNA复制起点完全位于腺病毒反向末端重复序列的前67个碱基对之内。该区域可进一步细分为两个功能域：一个最小复制起点和一个相邻的辅助区域，该辅助区域可将复制效率提高100倍以上。最小起点占据前18至21个碱基对，包括所有腺病毒血清型之间保守的序列。相邻的辅助区域延伸至核苷酸36之后，但不超过核苷酸67，并包含核因子I的结合位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/321857/03184d498494/nar00308-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/321857/28c3943630dc/nar00308-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/321857/03184d498494/nar00308-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/321857/28c3943630dc/nar00308-0177-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96b5/321857/03184d498494/nar00308-0178-a.jpg

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本文引用的文献

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Conserved sequences at the origin of adenovirus DNA replication.腺病毒DNA复制起始点的保守序列。
J Virol. 1982 Nov;44(2):530-7. doi: 10.1128/JVI.44.2.530-537.1982.
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Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1397-401. doi: 10.1073/pnas.90.4.1397.
7
The adenovirus DNA binding protein enhances intermolecular DNA renaturation but inhibits intramolecular DNA renaturation.腺病毒DNA结合蛋白增强分子间DNA复性，但抑制分子内DNA复性。
Nucleic Acids Res. 1993 Jun 11;21(11):2591-8. doi: 10.1093/nar/21.11.2591.
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Protein-primed DNA replication: role of inverted terminal repeats in the Escherichia coli bacteriophage PRD1 life cycle.蛋白质引发的DNA复制：反向末端重复序列在大肠杆菌噬菌体PRD1生命周期中的作用。
J Virol. 1993 Aug;67(8):4696-703. doi: 10.1128/JVI.67.8.4696-4703.1993.
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Adenovirus origin of DNA replication: sequence requirements for replication in vitro.腺病毒DNA复制的起源：体外复制的序列要求
Mol Cell Biol. 1987 Feb;7(2):864-74. doi: 10.1128/mcb.7.2.864-874.1987.
10
Analysis of nuclear factor I binding to DNA using degenerate oligonucleotides.使用简并寡核苷酸分析与DNA结合的核因子I
Nucleic Acids Res. 1986 Nov 25;14(22):9117-32. doi: 10.1093/nar/14.22.9117.
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Structure and function of the adenovirus origin of replication.腺病毒复制起点的结构与功能。
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