Ishii S, Xu Y H, Stratton R H, Roe B A, Merlino G T, Pastan I
Proc Natl Acad Sci U S A. 1985 Aug;82(15):4920-4. doi: 10.1073/pnas.82.15.4920.
The promoter region of the epidermal growth factor (EGF) receptor has been identified by in vitro transcription using EGF receptor genomic DNA fragments as template and by primer extension and nuclease S1 mapping using EGF receptor mRNA. Six transcriptional start sites were identified. DNA sequence analysis shows that the promoter region contains neither a "TATA box" nor a "CAAT box," has an extremely high G+C content (88%), and contains five CCGCCC repeats and four (TCC)TCCTCCTCC repeats. This promoter region is situated close to or within a DNase I-hypersensitive site in A431 human epidermoid carcinoma cells, which overproduce the EGF receptor. The EGF receptor gene promoter has some resemblance to the promoter of the hydroxymethylglutaryl-CoA reductase gene and the early promoter of simian virus 40. This similarity may offer a clue to the mechanism by which the receptor gene is regulated.
利用表皮生长因子（EGF）受体基因组DNA片段作为模板进行体外转录，以及利用EGF受体mRNA进行引物延伸和核酸酶S1作图，已确定了EGF受体的启动子区域。确定了六个转录起始位点。DNA序列分析表明，启动子区域既不包含“TATA盒”也不包含“CAAT盒”，G+C含量极高（88%），并包含五个CCGCCC重复序列和四个（TCC）TCCTCCTCC重复序列。该启动子区域位于过度产生EGF受体的A431人表皮样癌细胞中一个DNase I超敏位点附近或之内。EGF受体基因启动子与羟甲基戊二酰辅酶A还原酶基因的启动子以及猿猴病毒40的早期启动子有一些相似之处。这种相似性可能为受体基因的调控机制提供线索。
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