De Larco J E, Pigott D A, Lazarus J A
Proc Natl Acad Sci U S A. 1985 Aug;82(15):5015-9. doi: 10.1073/pnas.82.15.5015.
Cells derived from a human melanoma strain by low-serum selection in monolayer were found to be capable of growth in semisolid medium, forming colonies ranging from tight to loose or dispersed. These phenotypes were found to be stable after cloning and retesting. Examination of the biological activities of ectopic peptides produced by a clone that gives rise to loose dispersed colonies revealed the presence of a transforming growth factor beta (TGF-beta; apparent Mr 14,700) and a single TGF-alpha species produced at an unusually high concentration (2.2 ng/ml). Coeluted with the TGF-alpha at approximately 22,500 was a mitogenic activity and a previously undescribed activity capable of modulating the phenotype of induced normal rat kidney fibroblast (NRK-49F) colonies in semisolid medium. This activity can modulate the usual tight colony to express a loose or dispersed phenotype characteristic of the producer cells. Both the possible role these ectopic peptides play in the expression of the transformed phenotype by the tumor cells producing them and the possible correlation between the Mr 22,500 epidermal growth factor-like peptide released by this particular tumor line and high molecular weight epidermal growth factor-like peptides found in the urine of cancer patients are discussed.
通过单层低血清筛选从人黑色素瘤株衍生的细胞被发现能够在半固体培养基中生长，形成从紧密到松散或分散的菌落。这些表型在克隆和重新测试后被发现是稳定的。对产生松散分散菌落的克隆所产生的异位肽的生物活性进行检测，发现存在一种转化生长因子β（TGF-β；表观分子量14,700）和一种以异常高浓度（2.2 ng/ml）产生的单一TGF-α。在大约22,500处与TGF-α共洗脱的是一种促有丝分裂活性和一种以前未描述的活性，该活性能够调节半固体培养基中诱导的正常大鼠肾成纤维细胞（NRK-49F）菌落的表型。这种活性可以调节通常紧密的菌落，使其表达出产生这些菌落的细胞所特有的松散或分散表型。讨论了这些异位肽在产生它们的肿瘤细胞转化表型表达中可能发挥的作用，以及该特定肿瘤系释放的分子量22,500的表皮生长因子样肽与癌症患者尿液中发现的高分子量表皮生长因子样肽之间可能的相关性。
