Salih Mutaz E, Aqel Ahmad, Abdulkhair Babiker Y, Alothman Zeid A, Abdulaziz Mohamad A, Badjah-Hadj-Ahmed A Yacine
Chemistry Department, College of Science, Sudan University for Science and Technology, Khartoum, Sudan.
Department of Chemistry-Hurrymilla, College of Science and Humanities, Shaqra University, Shaqra, Saudi Arabia.
J Chromatogr Sci. 2018 Oct 1;56(9):819-827. doi: 10.1093/chromsci/bmy058.
Recently, analytical separation techniques have the potential toward green approaches to reduce the environmental impact. This study focuses on the development of an analytical method for the determination of paracetamol and chlorzoxazone in their pharmaceutical combination. The separation was achieved using a home-made capillary column (0.10 mm i.d. × 200 mm length) filled with porous cross-linked hexyl polymethacrylate as monolithic stationary phase. The method proved to be simple, fast, sensitive, efficient, cost-effective and green approach due to the combination of the amazing properties of a monolithic material and a miniaturized liquid chromatography, which would be considered as a step toward reducing the analytical costs and the environmental impact of chromatographic applications. Both components were detected using a 3-nL nano-UV cell fixed at 270 nm wavelength. The optimized mobile phase was composed of 1% aqueous formic acid solution and acetonitrile at 40:60 ratio, 1.0 μL/min flow rate, 4.0 nL injection volume and 50°C column temperature. Under the optimized conditions, paracetamol and chlorzoxazone have been separated in about 6.5 min with chromatographic resolution of 2.37. The prepared column and the analysis method was fully validated and compared with other reported works. All findings allow to conclude that the prepared column and proposed method are applicable for quality control and routine analysis of the two drugs.
最近，分析分离技术有朝着绿色方法发展以减少环境影响的潜力。本研究聚焦于开发一种用于测定药物组合中对乙酰氨基酚和氯唑沙宗的分析方法。分离是使用一根自制的毛细管柱（内径0.10 mm×长度200 mm）实现的，该柱填充有多孔交联聚甲基丙烯酸己酯作为整体固定相。由于整体材料的惊人特性与微型液相色谱相结合，该方法被证明是简单、快速、灵敏、高效、经济高效且绿色的方法，这将被视为朝着降低分析成本和色谱应用的环境影响迈出的一步。使用固定在270 nm波长的3 nL纳米紫外池检测两种组分。优化后的流动相由1%甲酸水溶液和乙腈按40:60的比例组成，流速为1.0 μL/min，进样体积为4.0 nL，柱温为50°C。在优化条件下，对乙酰氨基酚和氯唑沙宗在约6.5分钟内分离，色谱分辨率为2.37。所制备的柱和分析方法经过了全面验证，并与其他已报道的工作进行了比较。所有结果表明所制备的柱和所提出的方法适用于这两种药物的质量控制和常规分析。
