Agbalika F, Wullenweber M, Prévot J
Zentralbl Bakteriol Mikrobiol Hyg B. 1985 May;180(5-6):534-9.
Samples of activated sludge were taken in three different treatment plants near Nancy (France) and in West Berlin (F.R.G.), and were investigated for their content of rotaviruses. After elution with beef extract (0.25%, pH 7.3) and ultrasonication, the samples were concentrated by protamine flocculation and, in a few cases, in parallel by ultracentrifugation. A commercially available ELISA test kit (Rotazyme, Abbott Diagnostics) was routinely used for rotavirus detection. Although both the Rotazyme and a laboratory-designed ELISA gave positive results for the suldge concentrates, confirmation with a blocking test and electron microscope (EM) was negative in all cases so far investigated. Therefore, the exclusive use of ELISA cannot be recommended for similar investigations as it may lead to false positive results. In this light, an additional verification step with neutralizing antibodies should be included by the ELISA kits' manufacturers to enable a quick confirmation of the user's results.
在法国南锡附近和联邦德国西柏林的三个不同污水处理厂采集活性污泥样本，检测其中轮状病毒的含量。用牛肉浸膏（0.25%，pH 7.3）洗脱并超声处理后，样本通过鱼精蛋白絮凝法进行浓缩，少数情况下同时采用超速离心法。常规使用市售ELISA检测试剂盒（Rotazyme，雅培诊断公司）检测轮状病毒。尽管Rotazyme试剂盒和实验室设计的ELISA检测均显示污泥浓缩物呈阳性，但到目前为止，在所有已调查的案例中，通过阻断试验和电子显微镜（EM）进行的确认均为阴性。因此，不建议在类似调查中仅使用ELISA，因为它可能导致假阳性结果。鉴于此，ELISA试剂盒制造商应增加一个用中和抗体进行的额外验证步骤，以便快速确认用户的检测结果。
