Lin Y-C, Hall A C, Simpson A H R W
Department of Orthopaedic Surgery, University of Edinburgh, The Chancellor's Building, 49 Little France Crescent, Edinburgh, EH16 4SB, UK and Taipei Medical University-Shuang Ho Hospital, Zhonghe District, New Taipei City, 23561, Taiwan.
Centre for Integrative Physiology, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh EH8 9XD, UK.
Bone Joint Res. 2018 May 5;7(3):205-212. doi: 10.1302/2046-3758.73.BJR-2017-0320. eCollection 2018 Mar.
The purpose of this study was to create a novel organ culture model for evaluating the effects of static and dynamic load on cartilage.
The metatarsophalangeal joints of 12 fresh cadaveric bovine feet were skinned and dissected aseptically, and cultured for up to four weeks. Dynamic movement was applied using a custom-made machine on six joints, with the others cultured under static conditions. Chondrocyte viability and matrix glycosaminoglycan (GAG) content were evaluated by the cell viability probes, 5-chloromethylfluorescein diacetate (CMFDA) and propidium iodide (PI), and dimethylmethylene blue (DMMB) assay, respectively.
Chondrocyte viability in the static model decreased significantly from 89.9% (sd 2.5%) (Day 0) to 66.5% (sd 13.1%) (Day 28), 94.7% (sd 1.1%) to 80. 9% (sd 5.8%) and 80.1% (sd 3.0%) to 46.9% (sd 8.5%) in the superficial quarter, central half and deep quarter of cartilage, respectively (p < 0.001 in each zone; one-way analysis of variance). The GAG content decreased significantly from 6.01 μg/mg (sd 0.06) (Day 0) to 4.71 μg/mg (sd 0.06) (Day 28) (p < 0.001; one-way analysis of variance). However, with dynamic movement, chondrocyte viability and GAG content were maintained at the Day 0 level over the four-week period without a significant change (chondrocyte viability: 92.0% (sd 4.0%) (Day 0) to 89.9% (sd 0.2%) (Day 28), 93.1% (sd 1.5%) to 93.8% (sd 0.9%) and 85.6% (sd 0.8%) to 84.0% (sd 2.9%) in the three corresponding zones; GAG content: 6.18 μg/mg (sd 0.15) (Day 0) to 6.06 μg/mg (sd 0.09) (Day 28)).
Dynamic joint movement maintained chondrocyte viability and cartilage GAG content. This long-term whole joint culture model could be of value in providing a more natural and controlled platform for investigating the influence of joint movement on articular cartilage, and for evaluating novel therapies for cartilage repair.: Y-C. Lin, A. C. Hall, A. H. R. W. Simpson. A novel organ culture model of a joint for the evaluation of static and dynamic load on articular cartilage. 2018;7:205-212. DOI: 10.1302/2046-3758.73.BJR-2017-0320.
本研究的目的是创建一种新型器官培养模型，用于评估静态和动态负荷对软骨的影响。
对12只新鲜牛尸体足部的跖趾关节进行去皮肤和无菌解剖，并培养长达四周。使用定制机器对六个关节施加动态运动，其他关节在静态条件下培养。分别通过细胞活力探针5-氯甲基荧光素二乙酸酯（CMFDA）和碘化丙啶（PI）以及二甲基亚甲蓝（DMMB）测定法评估软骨细胞活力和基质糖胺聚糖（GAG）含量。
静态模型中软骨细胞活力在第28天显著下降，在软骨的浅表层从89.9%（标准差2.5%）（第0天）降至66.5%（标准差13.1%），在中间层从94.7%（标准差1.1%）降至80.9%（标准差5.8%），在深层从80.1%（标准差3.0%）降至46.9%（标准差8.5%）（每个区域p<0.001；单因素方差分析）。GAG含量从6.01μg/mg（标准差0.06）（第0天）显著降至4.71μg/mg（标准差0.06）（第28天）（p<0.001；单因素方差分析）。然而，在动态运动下，软骨细胞活力和GAG含量在四周内维持在第0天的水平，无显著变化（软骨细胞活力：在三个相应区域分别为92.0%（标准差4.0%）（第0天）至89.9%（标准差0.2%）（第28天）、93.1%（标准差1.5%）至93.8%（标准差0.9%）、85.6%（标准差0.8%）至84.0%（标准差2.9%）；GAG含量：6.18μg/mg（标准差0.15）（第0天）至6.06μg/mg（标准差0.09）（第28天））。
动态关节运动可维持软骨细胞活力和软骨GAG含量。这种长期的全关节培养模型可能有助于提供一个更自然且可控的平台，用于研究关节运动对关节软骨的影响，以及评估软骨修复的新疗法。：林Y-C、霍尔A.C.、辛普森A.H.R.W.。一种用于评估静态和动态负荷对关节软骨影响的新型关节器官培养模型。2018年；7：205 - 212。DOI：10.1302/2046 - 3758.73.BJR - 2017 - 0320 。
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