Kazemi D, Shams Asenjan K, Dehdilani N, Parsa H
Department of Veterinary Clinical Sciences, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Tabriz University of Medical Sciences, Haematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Bone Joint Res. 2017 Feb;6(2):98-107. doi: 10.1302/2046-3758.62.BJR-2016-0188.R1.
Mesenchymal stem cells have the ability to differentiate into various cell types, and thus have emerged as promising alternatives to chondrocytes in cell-based cartilage repair methods. The aim of this experimental study was to investigate the effect of bone marrow derived mesenchymal stem cells combined with platelet rich fibrin on osteochondral defect repair and articular cartilage regeneration in a canine model.
Osteochondral defects were created on the medial femoral condyles of 12 adult male mixed breed dogs. They were either treated with stem cells seeded on platelet rich fibrin or left empty. Macroscopic and histological evaluation of the repair tissue was conducted after four, 16 and 24 weeks using the International Cartilage Repair Society macroscopic and the O'Driscoll histological grading systems. Results were reported as mean and standard deviation (sd) and compared at different time points between the two groups using the Mann-Whitney U test, with a value < 0.05 considered statistically significant.
Higher cumulative macroscopic and histological scores were observed in stem cell treated defects throughout the study period with significant differences noted at four and 24 weeks (9.25, sd 0.5 7.25, sd 0.95, and 10, sd 0.81 7.5, sd 0.57; p < 0.05) and 16 weeks (16.5, sd 4.04 11, sd 1.15; p < 0.05), respectively. Superior gross and histological characteristics were also observed in stem cell treated defects.
The use of autologous culture expanded bone marrow derived mesenchymal stem cells on platelet rich fibrin is a novel method for articular cartilage regeneration. It is postulated that platelet rich fibrin creates a suitable environment for proliferation and differentiation of stem cells by releasing endogenous growth factors resulting in creation of a hyaline-like reparative tissue. D. Kazemi, K. Shams Asenjan, N. Dehdilani, H. Parsa. Canine articular cartilage regeneration using mesenchymal stem cells seeded on platelet rich fibrin: Macroscopic and histological assessments. 2017;6:98-107. DOI: 10.1302/2046-3758.62.BJR-2016-0188.R1.
间充质干细胞具有分化为多种细胞类型的能力，因此在基于细胞的软骨修复方法中，已成为软骨细胞有前景的替代物。本实验研究的目的是在犬模型中研究骨髓来源的间充质干细胞与富血小板纤维蛋白联合应用对骨软骨缺损修复和关节软骨再生的影响。
在12只成年雄性杂种犬的内侧股骨髁上制造骨软骨缺损。它们要么用接种在富血小板纤维蛋白上的干细胞治疗，要么保持缺损状态。在4周、16周和24周后，使用国际软骨修复协会宏观评分系统和奥德里斯科尔组织学评分系统对修复组织进行宏观和组织学评估。结果以平均值和标准差（sd）报告，并使用曼-惠特尼U检验在两组的不同时间点进行比较，P值<0.05被认为具有统计学意义。
在整个研究期间，干细胞治疗的缺损处观察到更高的累积宏观和组织学评分，在4周和24周（9.25，sd 0.5对7.25，sd 0.95，以及10，sd 0.81对7.5，sd 0.57；P<0.05）以及16周（16.5，sd 4.04对11，sd 1.15；P<0.05）时均有显著差异。在干细胞治疗的缺损处还观察到更好的大体和组织学特征。
将自体培养扩增的骨髓来源间充质干细胞应用于富血小板纤维蛋白是一种关节软骨再生的新方法。据推测，富血小板纤维蛋白通过释放内源性生长因子为干细胞的增殖和分化创造了合适的环境，从而导致形成类似透明软骨的修复组织。D. 卡泽米、K. 沙姆斯·阿森扬、N. 德迪拉尼、H. 帕尔萨。使用接种在富血小板纤维蛋白上的间充质干细胞进行犬关节软骨再生：宏观和组织学评估。2017年；6：98 - 107。DOI：10.1302/2046 - 3758.6（2）.BJR - 2016 - 0188.R1 。
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