A high-performance liquid chromatographic technique that separates cellular retinol binding protein from cellular retinoic acid binding protein.
作者信息
Bonelli F C, De Luca L M
出版信息
Anal Biochem. 1985 May 15;147(1):251-7. doi: 10.1016/0003-2697(85)90035-1.
PMID:2992315
Abstract
A one-step procedure to detect cellular [3H]retinol and [3H]retinoic acid binding proteins (CRBP and CRABP) from rat testis cytosolic extract was devised. The procedure is based on anion-exchange high-performance liquid chromatography of the cytosolic fraction on columns of Mono Q, which permits elution of CRABP and CRBP at 12 and 22 min, respectively.
摘要
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