McGregor S, Easterday B C, Kaplan A S, Ben-Porat T
Am J Vet Res. 1985 Jul;46(7):1494-7.
A mutant of pseudorabies virus (PRV) deficient in thymidine kinase (TK-) activity was isolated and characterized. The mutant grew well in cell culture and did not revert to the thymidine kinase-positive phenotype. The PRV-TK- was not virulent when inoculated intranasally into 3-to 4-week-old pigs and could not be reactivated from the ganglia of these pigs by explantation and cocultivation with susceptible cells several weeks after virus inoculation. Pigs that had been exposed to PRV-TK- were immune to challenge exposure with a virulent strain of PRV. Furthermore, the challenge virus was not recovered from the ganglia of most of these pigs, indicating that colonization of the ganglia by a super-infecting virulent PRV strain was considerably reduced by vaccination.
分离并鉴定了一株胸苷激酶（TK-）活性缺陷的伪狂犬病病毒（PRV）突变体。该突变体在细胞培养中生长良好，且未回复到胸苷激酶阳性表型。将PRV-TK-经鼻接种到3至4周龄仔猪时无毒力，且在病毒接种数周后，通过外植和与易感细胞共培养，无法从这些仔猪的神经节中重新激活该病毒。接触过PRV-TK-的仔猪对强毒株PRV的攻击具有免疫力。此外，在大多数这些仔猪的神经节中未检测到攻击病毒，这表明接种疫苗可显著减少超感染强毒PRV毒株在神经节中的定植。
