McGill Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty of Dentistry, McGill University, Montreal, QC, Canada.
Oral Pathology Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
Oral Dis. 2018 Nov;24(8):1477-1483. doi: 10.1111/odi.12920. Epub 2018 Jul 10.
The human salivary gland (HSG) cell line, labeled as a submandibular ductal cell line, is commonly used as in vitro models to study radiation therapy, Sjögren's syndrome, pleomorphic adenoma, mucocele, epithelial-to-mesenchymal transition, and epigenetics. However, the American Type Culture Collection (ATCC) has recently released a list of cross-contaminated cell lines that included HSG. Despite this notice, some research laboratories still use HSG as a salivary cell model. Therefore, this study examined the authenticity of HSG sampled from three different laboratories.
DNA was extracted from HSG and additional salivary cell lines (NS-SV-AC, NS-SV-DC, A253, HSY) and submitted for cell line authentication with short tandem repeat (STR) analysis.
All HSG samples had STR profiles indicating >80% match with HeLa in both the ATCC and Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) databases. This confirmed that HSG sampled from three different laboratories and HSY shared a common ancestry (host) with HeLa, whereas NS-SV-AC, NS-SV-DC, and A253 had unique STR profiles.
Short tandem repeat analysis revealed that HSG was contaminated by the HeLa cell line. Furthermore, because genotyping of the original HSG cell line was not performed during its establishment, it will be difficult to authenticate an uncontaminated sample of HSG.
人唾液腺（HSG）细胞系，标记为下颌下腺导管细胞系，通常被用作体外模型来研究放射治疗、干燥综合征、多形性腺瘤、黏液囊肿、上皮间质转化和表观遗传学。然而，美国模式培养物集存库（ATCC）最近公布了一份交叉污染细胞系清单，其中包括 HSG。尽管有此通知，一些研究实验室仍将 HSG 用作唾液腺细胞模型。因此，本研究检查了来自三个不同实验室的 HSG 的真实性。
从 HSG 和其他唾液腺细胞系（NS-SV-AC、NS-SV-DC、A253、HSY）中提取 DNA，并进行短串联重复序列（STR）分析以进行细胞系鉴定。
所有 HSG 样本的 STR 图谱均表明，在 ATCC 和德国微生物和细胞培养物收藏（DSMZ）数据库中，与 HeLa 的匹配度均超过 80%。这证实了来自三个不同实验室的 HSG 和 HSY 与 HeLa 具有共同的祖先（宿主），而 NS-SV-AC、NS-SV-DC 和 A253 则具有独特的 STR 图谱。
短串联重复序列分析显示 HSG 被 HeLa 细胞系污染。此外，由于在其建立过程中未对原始 HSG 细胞系进行基因分型，因此很难鉴定 HSG 的未污染样本。
