Arts C J, Koppeschaar H P, Veeman W, Thijssen J H
Ann Clin Biochem. 1985 May;22 ( Pt 3):247-56. doi: 10.1177/000456328502200306.
A direct radioimmunoassay for the determination of ACTH in a small volume of serum was developed using a commercially available antiserum and tracer. A single-stage radioimmunoassay with an incubation period of 4 days gave reliable and reproducible ACTH values. In the incubation medium 0.1% 2-mercaptoethanol was used as the antioxidant. During a 15-month period the assay has been used routinely in the laboratory. The coefficient of variation was less than 10% in the physiological and pathological range. A normal range of 12 to 60 ng/L was found. The reliability of the assay is demonstrated by results obtained in patients with disorders of the pituitary-adrenal axis.
采用市售抗血清和示踪剂建立了一种直接放射免疫分析法，用于测定少量血清中的促肾上腺皮质激素（ACTH）。单阶段放射免疫分析法的孵育期为4天，可得出可靠且可重复的ACTH值。在孵育介质中，使用0.1%的2-巯基乙醇作为抗氧化剂。在15个月的时间里，该分析法一直在实验室中常规使用。在生理和病理范围内，变异系数小于10%。发现正常范围为12至60 ng/L。垂体-肾上腺轴紊乱患者的检测结果证明了该分析法的可靠性。
