Research Institute of Wastes and Soil Remediation, Shanghai Academy of Environmental Sciences, Shanghai, People's Republic of China.
Environ Toxicol Chem. 2018 Oct;37(10):2560-2565. doi: 10.1002/etc.4214. Epub 2018 Aug 23.
Because of increasing concerns about its toxic effects, bisphenol A (BPA) has been gradually replaced in industrial applications by analogs such as bisphenol S (BPS). Few comparative toxicity evaluations of bisphenol analogs have been done. In the present study, 72-h exposure in L1 larvae of the model animal Caenorhabditis elegans was used to evaluate low-concentration BPS toxicity. Multiple indicators at the physiological, biochemical, and molecular levels were tested. At the physiological level, BPS exposure resulted in significantly negative effects at treatments >1 µM, with head thrash being the most sensitive endpoint. At the biochemical level, BPS exposure induced no significant oxidative stress, but significantly increased apoptosis at 1 µM. At the molecular level, BPS exposure induced small but significant variations in most stress-related gene expressions at all doses. In addition, the transgenic nematode TJ375 cell line with the green fluorescent protein-based reporter hsp-16.2 was used to determine stress responses; it was found that TJ375 was not sensitive to BPS exposure. Compared with the effects of BPA shown in our previous 2016 study, the overall results showed that BPS was less noxious to C. elegans than BPA. These toxicity data for BPS could provide a foundation to evaluate the comparative toxicity of BPA alternatives. Environ Toxicol Chem 2018;37:2560-2565. © 2018 SETAC.
由于对其毒性作用的担忧日益增加，双酚 A（BPA）在工业应用中已逐渐被类似物如双酚 S（BPS）取代。目前对双酚类似物的比较毒性评价研究较少。本研究采用模式动物秀丽隐杆线虫 L1 幼虫 72 h 暴露实验，评估低浓度 BPS 的毒性。在生理、生化和分子水平上测试了多个指标。在生理水平上，BPS 暴露在 >1µM 时会产生显著的负面影响，头部摆动是最敏感的终点。在生化水平上，BPS 暴露未引起明显的氧化应激，但在 1µM 时显著增加了凋亡。在分子水平上，BPS 暴露在所有剂量下都引起了大多数应激相关基因表达的微小但显著的变化。此外，还使用了带有绿色荧光蛋白报告基因 hsp-16.2 的转基因线虫 TJ375 细胞系来测定应激反应；结果发现 TJ375 对 BPS 暴露不敏感。与我们 2016 年的研究中 BPA 的影响相比，总体结果表明 BPS 对秀丽隐杆线虫的毒性比 BPA 低。这些 BPS 的毒性数据可为评估 BPA 替代物的比较毒性提供基础。环境毒理化学 2018；37：2560-2565。©2018 SETAC。
