菜豆假单胞菌丁香致病变种粘粒克隆文库的构建及通过功能互补分离基因。
Construction of a cosmid clone library of Pseudomonas syringae pv. phaseolicola and isolation of genes by functional complementation.
作者信息
Ehrenshaft M, Mills D
出版信息
Appl Environ Microbiol. 1985 Jul;50(1):169-71. doi: 10.1128/aem.50.1.169-171.1985.
Abstract
A genomic library constructed from a wild-type strain of Pseudomonas syringae pv. phaseolicola in the broad-host-range cosmid vector pVK102 was used to isolate wild-type genes by complementation of Tn5-induced auxotrophic mutants. Selection pressure was required for maintenance of the vector and members of the library in strains of P. syringae.
摘要
用广宿主黏粒载体pVK102构建丁香假单胞菌菜豆致病变种野生型菌株的基因组文库，通过Tn5诱导的营养缺陷型突变体的互补作用来分离野生型基因。在丁香假单胞菌菌株中维持载体和文库成员需要选择压力。
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