构建用于丁香假单胞菌丁香致病变种高频转化的稳定穿梭载体。
Construction of a stable shuttle vector for high-frequency transformation in Pseudomonas syringae pv. syringae.
作者信息
Mukhopadhyay P, Mukhopadhyay M, Mills D
机构信息
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.
出版信息
J Bacteriol. 1990 Jan;172(1):477-80. doi: 10.1128/jb.172.1.477-480.1990.
Abstract
A cryptic 80.3-kilobase plasmid, pOSU900, in Pseudomonas syringae pv. syringae strain J900 could be cured by treatment with mitomycin without affecting the pathogenicity of J900 on the host, Phaseolus vulgaris L. The replication region of pOSU900 was identified, subcloned, and modified for construction of a high-copy cloning vector. This vector could be transformed into Pseudomonas strains with high efficiency (ca. 10(6) transformants per microgram of DNA) and was very stable during growth of the host bacteria in planta.
摘要
丁香假单胞菌丁香致病变种J900中的一个80.3千碱基的隐蔽性质粒pOSU900，可用丝裂霉素处理治愈，且不影响J900对宿主菜豆的致病性。鉴定了pOSU900的复制区域，进行亚克隆并加以修饰，用于构建高拷贝克隆载体。该载体可高效转化到假单胞菌菌株中（约每微克DNA有10⁶个转化子），并且在宿主细菌于植物体内生长期间非常稳定。
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