Bhattacharyya L, Freedman J H, Brewer C F, Brown R D, Koenig S H
Arch Biochem Biophys. 1985 Aug 1;240(2):820-6. doi: 10.1016/0003-9861(85)90091-8.
The lentil isolectins, CMLcH A and CMLcH B, and pea isolectins, CMPSA A and CMPSA B, are compared in terms of their spectroscopic and saccharide binding properties. The paramagnetic contribution to the solvent proton magnetic relaxation dispersion profiles of solutions of the isolectins of each protein are found to be essentially identical. Electron paramagnetic resonance spectra suggest a high degree of octahedral symmetry at the Mn2+ site for both pairs of isolectins. The near-ultraviolet absorption spectra of CMLcH A and CMLcH B are identical, as are the spectra of CMPSA A and CMPSA B. Carbohydrate binding activities of the isolectins of each protein are compared using hemagglutination, precipitation, and precipitation-inhibition assays, and are found to be identical, although the activities of CMLcH and CMPSA differ somewhat. These results demonstrate that the spectroscopic and saccharide binding properties of the isolectins of CMLcH are essentially identical, as are those of the isolectins of CMPSA, and suggest that native mixtures of the isolectins may be treated as single proteins in further studies.
对小扁豆异凝集素CMLcH A和CMLcH B以及豌豆异凝集素CMPSA A和CMPSA B的光谱性质和糖结合特性进行了比较。发现每种蛋白质的异凝集素溶液对溶剂质子磁弛豫分散谱的顺磁贡献基本相同。电子顺磁共振光谱表明，这两对异凝集素在Mn2+位点都具有高度的八面体对称性。CMLcH A和CMLcH B的近紫外吸收光谱相同，CMPSA A和CMPSA B的光谱也相同。使用血细胞凝集、沉淀和沉淀抑制试验比较了每种蛋白质的异凝集素的碳水化合物结合活性，发现它们是相同的，尽管CMLcH和CMPSA的活性略有不同。这些结果表明，CMLcH的异凝集素的光谱性质和糖结合特性基本相同，CMPSA的异凝集素也是如此，这表明在进一步的研究中，异凝集素的天然混合物可被视为单一蛋白质。
