College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei 071001, China.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100093, China.
Poult Sci. 2018 Oct 1;97(10):3405-3413. doi: 10.3382/ps/pey231.
In the current study, we sought to determine whether or not the endogenous retroviral ev21 influences feathering type of chickens, and if one mutation locus in the unoccupied repeat (UR) region can be used to predict the corresponding feathering type and genotype. The distribution of ev21 as well as the mutation locus in UR and occupied site (OR) regions was detected in HY-line gray progenitor (HYGP) 4 lines, HY-line brown (HYB) and Taihang chickens (TH). Furthermore, a detection method for the genotype resulting in late feathering (LF) phenotype was developed by double PCR using C line of HYGP, C line of Dawu progenitor, commercial line of HY-line gray (HYG) males, LF males of TH and Bashang long-tail chickens (BS). Results indicated that a product of 7590 bp from the long fragment amplification was observed to be a partial segment of ev21, and was linked with the LF phenotype in HYGP but not in HYB and TH chickens. A total of 2 of 35 males and 10 of 29 females of TH LF chickens were found to be ev21 negative. HaeIII RFLP mutations of 1450 bp of UR, 1440 bp of OR, and 538 bp in the UR and OR common region were analyzed, and genotypic features at the locus correlated with the feathering type phenotype in HYGP, but exhibited no significant effects in HYB and TH chickens. The cut-off of relative intensity of 857 and 1305 bp from the double PCR for distinction between homozygous and heterozygous LF males was 1.37. In conclusion, ev21 and the HaeIII RFLP patterns within the locus in UR cannot be used for prediction of feathering type phenotypes in Chinese heritage chickens. However, the partial duplication of PRLR and SPEF2 were able to predict the LF phenotype. Therefore, the double PCR detecting products of 857 and 1305 bp described herein could be used for the accurate identification of genotypes influencing feathering type.
在本研究中，我们旨在确定内源性逆转录病毒 ev21 是否影响鸡的羽毛类型，以及非占据重复（UR）区域中的一个突变位点是否可用于预测相应的羽毛类型和基因型。我们在 HY 系灰色原种鸡（HYGP）4 个品系、HY 系棕色（HYB）和太行鸡（TH）中检测了 ev21 的分布以及 UR 和占据位点（OR）区域中的突变位点。此外，我们还通过使用 HYGP 的 C 系、大悟原种的 C 系、HY 系灰色商品代公鸡（HYG）、TH 的 LF 公鸡和坝上长尾鸡（BS），开发了一种用于检测迟羽（LF）表型基因型的双重 PCR 检测方法。结果表明，长片段扩增的 7590 bp 产物被观察为 ev21 的部分片段，与 HYGP 的 LF 表型相关，但与 HYB 和 TH 鸡的 LF 表型无关。TH 的 LF 鸡中有 2 只公鸡和 10 只母鸡被发现为 ev21 阴性。分析了 UR 的 1450 bp、OR 的 1440 bp 和 UR 和 OR 公共区域的 538 bp 的 HaeIII RFLP 突变，以及该位点的基因型特征与 HYGP 的羽毛类型表型相关，但在 HYB 和 TH 鸡中无显著影响。双 PCR 区分 LF 公鸡纯合子和杂合子的 857 和 1305 bp 相对强度的截止值为 1.37。总之，ev21 和 UR 中该基因座的 HaeIII RFLP 模式不能用于预测中国地方鸡种的羽毛类型表型。然而，PRLR 和 SPEF2 的部分重复可用于预测 LF 表型。因此，本文所述的检测 857 和 1305 bp 产物的双重 PCR 可用于准确鉴定影响羽毛类型的基因型。
