Han Yu-heng, Lai Xing-huan, Le Zi-wei, Hua Zi-chun
Yao Xue Xue Bao. 2016 Sep;51(9):1417-22.
Salmonella is a gram-negative bacterium that has an ability of tumor-targeting growth and proliferation. Attenuated Salmonella VNP20009 is a virulence genes-knockout bacterial strain based on Salmonella typhimurium, and it has an advantage of good therapeutic effect and low toxicity. One of the mechanisms of anti-tumor effect of VNP20009 is the induction of inflammatory reaction within tumor tissues. We used B16F10 melanoma model to investigate the mechanism of the anti-tumor effect of VNP20009. VNP20009 treatment effectively inhibited tumor growth and promoted the apoptosis and necrosis of tumor cells. VNP20009 increased the accumulation or infiltration of CD8(+) T cells and CD11b(+) monocytes within tumor tissue by raising the level of immune response and thus, induce the production of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) to kill tumor cells by breaking the immuno-evasion barrier in the tumor microenvironment.
沙门氏菌是一种革兰氏阴性细菌，具有肿瘤靶向生长和增殖的能力。减毒沙门氏菌VNP20009是一种基于鼠伤寒沙门氏菌的毒力基因敲除菌，具有治疗效果好、毒性低的优点。VNP20009抗肿瘤作用的机制之一是诱导肿瘤组织内的炎症反应。我们使用B16F10黑色素瘤模型来研究VNP20009抗肿瘤作用的机制。VNP20009治疗有效抑制肿瘤生长，促进肿瘤细胞凋亡和坏死。VNP20009通过提高免疫反应水平增加肿瘤组织内CD8(+) T细胞和CD11b(+)单核细胞的聚集或浸润，从而诱导肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）的产生，通过打破肿瘤微环境中的免疫逃逸屏障来杀死肿瘤细胞。
