二价阳离子对小鼠肝细胞中促生长和促乳结合位点检测的影响。
Influence of divalent cations on the detection of somatogenic and lactogenic binding sites in mouse liver cells.
作者信息
Ciccia-Torres G N, Dellacha J M
出版信息
Biochem J. 1985 Jun 15;228(3):761-4. doi: 10.1042/bj2280761.
Abstract
Specific binding of 125I-labelled human somatotropin was demonstrated in isolated hepatocytes from male mice. In the presence of divalent cations (Ca2+ and Mg2+) the binding of 125I-labelled human somatotropin was competitive with ovine prolactin. Scatchard analysis of competition data indicated a KD of 1.4 +/- 0.2 nM and a binding capacity of 13 000 +/- 2000 sites/cell. In the absence of divalent cations and in the presence of EDTA, human and bovine somatotropins were found to be equally effective to displace bound 125I-labelled human somatotropin, while ovine prolactin showed a weak competition. In this case, the binding capacity was 8400 +/- 1500 sites/cell and the KD was 1.1 +/- 0.1 nM.
摘要
在雄性小鼠分离的肝细胞中证实了125I标记的人生长激素的特异性结合。在二价阳离子（Ca2+和Mg2+）存在的情况下，125I标记的人生长激素的结合与羊催乳素具有竞争性。对竞争数据的Scatchard分析表明，解离常数（KD）为1.4±0.2 nM，结合容量为13000±2000个位点/细胞。在不存在二价阳离子且存在乙二胺四乙酸（EDTA）的情况下，发现人和牛生长激素在置换结合的125I标记人生长激素方面同样有效，而羊催乳素的竞争性较弱。在这种情况下，结合容量为8400±1500个位点/细胞，KD为1.1±0.1 nM。
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