Greene R M, Lloyd M R
Biochem Biophys Res Commun. 1985 Aug 15;130(3):1037-43. doi: 10.1016/0006-291x(85)91720-6.
The influence of epidermal growth factor (EGF) on the ability of murine embryonic palate mesenchymal (MEPM) cells to be stimulated to synthesize cAMP and prostaglandins was investigated. Preincubation of MEPM cells with EGF enhanced, in a dose-dependent fashion, (1) the responsiveness of MEPM cells to prostaglandin E1-induced elevation of intracellular levels of cAMP, and (2) the responsiveness of cells to calcium ionophore (A23187) and melittin-induced synthesis of prostaglandins E2 and F2 alpha. Hormonal responsiveness of MEPM cells to EGF, prostaglandins and cAMP has been implicated as being involved in controlling various aspects of normal oro-facial development. We show here that EGF can potentiate hormonal responsiveness of these cells and thus allows consideration of EGF as a factor which may modulate hormonally regulated craniofacial growth and differentiation.
研究了表皮生长因子（EGF）对小鼠胚胎腭间充质（MEPM）细胞被刺激合成环磷酸腺苷（cAMP）和前列腺素能力的影响。用EGF对MEPM细胞进行预孵育，以剂量依赖的方式增强了：（1）MEPM细胞对前列腺素E1诱导的细胞内cAMP水平升高的反应性；（2）细胞对钙离子载体（A23187）和蜂毒肽诱导的前列腺素E2和F2α合成的反应性。MEPM细胞对EGF、前列腺素和cAMP的激素反应性被认为参与了正常口面部发育的各个方面。我们在此表明，EGF可增强这些细胞的激素反应性，因此可以认为EGF是一种可能调节激素调控的颅面生长和分化的因子。
