Mills I, Fain J N
Biochem Biophys Res Commun. 1985 Aug 15;130(3):1059-65. doi: 10.1016/0006-291x(85)91723-1.
Insulin inhibition of lipolysis in the presence of forskolin was reversed by a four hour exposure of adipocytes to pertussis toxin. In contrast, the antilipolytic action of insulin against lipolysis due to theophylline was unaffected by pertussis toxin as was the ability of insulin to lower cyclic AMP in the presence of either forskolin or theophylline. The stimulation of adenylate cyclase by norepinephrine in crude plasma membranes obtained from rat adipocytes was inhibited by N6-(Phenylisopropyl)adenosine (PIA) and abolished by pretreating rat adipocytes with pertussis toxin. The stimulation of glucose metabolism by insulin was not altered by pertussis toxin pretreatment of rat adipocytes. These findings suggest that pertussis toxin selectively abolishes the antilipolytic effect of insulin in the presence of forskolin through a cyclic AMP independent mechanism.
在存在福斯高林的情况下，胰岛素对脂肪分解的抑制作用在脂肪细胞暴露于百日咳毒素4小时后被逆转。相比之下，胰岛素对由茶碱引起的脂肪分解的抗脂解作用不受百日咳毒素影响，并且在存在福斯高林或茶碱的情况下，胰岛素降低环磷酸腺苷的能力也不受影响。从大鼠脂肪细胞获得的粗质膜中，去甲肾上腺素对腺苷酸环化酶的刺激作用被N6-（苯异丙基）腺苷（PIA）抑制，并在大鼠脂肪细胞用百日咳毒素预处理后被消除。胰岛素对葡萄糖代谢的刺激作用在大鼠脂肪细胞用百日咳毒素预处理后未改变。这些发现表明，百日咳毒素通过一种不依赖环磷酸腺苷的机制，在存在福斯高林的情况下选择性地消除胰岛素的抗脂解作用。
