Tagliaferri P, Clair T, DeBortoli M E, Cho-Chung Y S
Biochem Biophys Res Commun. 1985 Aug 15;130(3):1193-200. doi: 10.1016/0006-291x(85)91741-3.
Factors that control cellular proliferation might do so by regulating quantitative expression of viral or cellular oncogenes. Since the growth regulatory effect of cAMP is well-known, the effect of cAMP on ras gene expression was examined on Ha-MuSV-transformed 13-3B-4 cells (NIH-3T3) grown in chemically defined serum-free medium. Treatment of cells with two classes of cAMP analogs which are selective for the two different cAMP-binding sites of type II protein kinase, in combination, synergistically inhibited both p21 ras protein synthesis and phenotypic transformation. The inhibition was also demonstrated with these analogs singly but at higher concentrations. The decrease in p21 synthesis was inversely correlated with an increase in the RII cAMP receptor protein, the regulatory subunit of type II protein kinase. These results suggest a role for cAMP and its receptor protein in the regulation of v-rasH oncogene expression.
控制细胞增殖的因素可能通过调节病毒或细胞癌基因的定量表达来实现。由于环磷酸腺苷（cAMP）的生长调节作用是众所周知的，因此在化学限定的无血清培养基中培养的Ha-MuSV转化的13-3B-4细胞（NIH-3T3）上检测了cAMP对ras基因表达的影响。用两类对II型蛋白激酶的两个不同cAMP结合位点具有选择性的cAMP类似物处理细胞，联合使用时可协同抑制p21 ras蛋白合成和表型转化。单独使用这些类似物时也能观察到抑制作用，但需要更高的浓度。p21合成的减少与II型蛋白激酶的调节亚基RII cAMP受体蛋白的增加呈负相关。这些结果表明cAMP及其受体蛋白在v-rasH癌基因表达的调节中起作用。
