Hazzard J T, Tollin G
Biochem Biophys Res Commun. 1985 Aug 15;130(3):1281-6. doi: 10.1016/0006-291x(85)91753-x.
The effects of complex formation with flavodoxin on the proton NMR spectrum of cytochrome c are to change the resonance frequencies and to increase the bandwidths of most of the low and high field heme, Met-80, and His-18 protons. These effects are, in general, more pronounced than has been reported for other cytochrome c complexes. The degree of line broadening for many heme related resonances suggests that complex formation induces changes in the cytochrome structure. These results provide the first spectroscopic evidence which corroborates the proposed model for the cytochrome c: flavodoxin complex (1-3).
与黄素氧还蛋白形成复合物对细胞色素c的质子核磁共振谱的影响是改变共振频率，并增加大多数低场和高场血红素、甲硫氨酸-80和组氨酸-18质子的带宽。一般来说，这些影响比其他细胞色素c复合物的报道更为明显。许多与血红素相关共振的谱线展宽程度表明复合物的形成会引起细胞色素结构的变化。这些结果提供了首个光谱学证据，证实了所提出的细胞色素c：黄素氧还蛋白复合物模型(1 - 3)。
