Vallano M L, Buckholz T M, DeLorenzo R J
Biochem Biophys Res Commun. 1985 Aug 15;130(3):957-63. doi: 10.1016/0006-291x(85)91708-5.
A protein fraction containing neurofilaments was prepared from rat brain cytosol by differential centrifugation and gel filtration chromatography. These preparations were enriched for a calcium/calmodulin-dependent kinase activity that phosphorylated endogenous neurofilament proteins. The enzyme incorporated approximately 1 mol PO4/mol of each neurofilament triplet polypeptide. These data suggest that a calmodulin-dependent kinase may mediate some of the effects of calcium on cytoskeletal function by phosphorylation of neurofilament proteins.
通过差速离心和凝胶过滤色谱法从大鼠脑细胞质中制备了一种含有神经丝的蛋白质组分。这些制剂富含一种钙/钙调蛋白依赖性激酶活性，该活性可使内源性神经丝蛋白磷酸化。该酶在每个神经丝三联体多肽中掺入约1摩尔磷酸根/摩尔。这些数据表明，钙调蛋白依赖性激酶可能通过神经丝蛋白的磷酸化介导钙对细胞骨架功能的某些影响。
