Sun N, Li Y, He J
Department of Thoracic Surgery, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
Zhonghua Yi Xue Za Zhi. 2018 Jun 5;98(21):1703-1706. doi: 10.3760/cma.j.issn.0376-2491.2018.21.016.
To analyze the potential clinical relevance of inhibitory immune checkpoint genes, including PD1, PD-L1, CTLA4, LAG3 and TIM3 in esophageal squamous cell carcinoma (ESCC). Expression were extracted from mRNA microarray data from 119 paired ESCC and adjacent normal tissues, which were collected from National Cancer Center/Chinese Academy of Medical Sciences between December 2005 and December 2007.Differential expression analyses and survival analyses were then performed to identify potential clinical relevance using R software. Expression of PD-L1, CTLA4, LAG3 and TIM3 were significantly upregulated in ESCC tissues compared with adjacent normal tissues (<0.05). CTLA4 was significantly correlated with tumor location and age (<0.05). PD1 and LAG3 were significantly associated with overall survival and were favorable prognostic factors in ESCC. Inhibitory immune checkpoint genes are commonly overexpressed in ESCC and are significantly associated with patients overall survival.These results provide important references in the application of immune checkpoints therapy in ESCC.
分析抑制性免疫检查点基因，包括程序性死亡受体1（PD1）、程序性死亡配体1（PD-L1）、细胞毒性T淋巴细胞相关抗原4（CTLA4）、淋巴细胞活化基因3（LAG3）和T细胞免疫球蛋白黏蛋白分子3（TIM3）在食管鳞状细胞癌（ESCC）中的潜在临床相关性。从2005年12月至2007年12月期间中国医学科学院肿瘤医院收集的119对ESCC组织及其癌旁正常组织的mRNA芯片数据中提取基因表达信息。然后使用R软件进行差异表达分析和生存分析，以确定潜在的临床相关性。与癌旁正常组织相比，ESCC组织中PD-L1、CTLA4、LAG3和TIM3的表达显著上调（P<0.05）。CTLA4与肿瘤位置和年龄显著相关（P<0.05）。PD1和LAG3与总生存期显著相关，是ESCC的有利预后因素。抑制性免疫检查点基因在ESCC中普遍过表达，且与患者总生存期显著相关。这些结果为免疫检查点疗法在ESCC中的应用提供了重要参考。
