Gaballah Ahmed Mohammed, Esawy Marwa Mohammed
Department of Clinical Pathology, Faculty of Medicine, Zagazig University, Egypt.
Diagn Microbiol Infect Dis. 2018 Oct;92(2):107-111. doi: 10.1016/j.diagmicrobio.2018.05.013. Epub 2018 May 21.
The measurement of antibodies against hepatitis C virus (HCV) is important to screen HCV infection. The aim of this study is to investigate the reliability of 2 commercially available anti-HCV antibody kits used in routine laboratory testing in Egypt. One thousand nine hundred and thirty-one serum samples were analyzed using 2 anti-HCV test systems: Cobas e 411® Elecsys Anti-HCVII and Vidas® Anti-HCV Biomerieux. Discrepant samples were tested using the recombinant immunoblot assay Innogenetics® INNO-LIA HCV Score. Overall agreement of the 2 tests was 94%. Following discrepant sample testing by LIA, sensitivity and specificity using Vidas were 94% and 99%, respectively, while those for Cobas were 97% and 96%, respectively. This study demonstrates superior specificity by Vidas and higher sensitivity by Cobas. Both methods are suitable for laboratory and/or blood screening programs. The concomitant use of a supplementary or confirmatory assay is necessary to compare the accuracy of HCV serological assays.
抗丙型肝炎病毒（HCV）抗体的检测对于筛查HCV感染至关重要。本研究旨在调查埃及常规实验室检测中使用的两种市售抗HCV抗体试剂盒的可靠性。使用两种抗HCV检测系统对1931份血清样本进行分析：Cobas e 411® Elecsys Anti-HCVII和Vidas® Anti-HCV Biomerieux。对结果不一致的样本使用重组免疫印迹法Innogenetics® INNO-LIA HCV Score进行检测。两种检测方法的总体一致性为94%。在通过LIA对结果不一致的样本进行检测后，Vidas的敏感性和特异性分别为94%和99%，而Cobas的敏感性和特异性分别为97%和96%。本研究表明Vidas具有更高的特异性，Cobas具有更高的敏感性。两种方法均适用于实验室和/或血液筛查项目。必须同时使用补充或确证检测来比较HCV血清学检测的准确性。
