Inaba M, Maede Y
Biochim Biophys Acta. 1985 Aug 27;818(2):267-70. doi: 10.1016/0005-2736(85)90567-x.
The (Na+ + K+)-ATPase from dog kidney and partially purified membranes from HK dog erythrocytes were labeled with [3H]ouabain, solubilized with C12E8 and analyzed by HPLC through a TSK-GEL G3000SW column in the presence of C12E8, Mg2+, HPO4(2-) and glycerol at 20-23 degrees C. The peaks of [3H]ouabain bound to the enzyme from dog kidney and HK dog erythrocyte membranes corresponded to each other with apparent molecular weights of 470 000-490 000. In addition, these bindings of [3H]ouabain to the (Na+ + K+)-ATPase were observed to be stable at 20-23 degrees C for at least 18 h after the solubilization.
用[³H]哇巴因标记犬肾的（Na⁺ + K⁺）-ATP酶和来自HK犬红细胞的部分纯化膜，用C₁₂E₈溶解，并在20 - 23℃下，于C₁₂E₈、Mg²⁺、HPO₄²⁻和甘油存在的条件下，通过TSK - GEL G3000SW柱进行高效液相色谱分析。与犬肾酶和HK犬红细胞膜结合的[³H]哇巴因峰彼此对应，表观分子量为470000 - 490000。此外，观察到这些[³H]哇巴因与（Na⁺ + K⁺）-ATP酶的结合在溶解后于20 - 23℃下至少18小时保持稳定。
