Namba Shihoko, Ikeda Fusao, Takaguchi Koichi, Shimomura Yasuyuki, Yasunaka Tetsuya, Okada Hiroyuki
Center for Innovative Clinical Medicine, Okayama University Hospital, Okayama 700-8558, Japan.
Acta Med Okayama. 2018 Jun;72(3):283-287. doi: 10.18926/AMO/56074.
We investigated whether a small amount of blood collected by fingertip blood sampling would be adequate in a mass examination for hepatitis virus infection in Japan. A cross-sectional survey was conducted at health fairs in Kasaoka City and Shodoshima Island, where participants took the hepatitis screening test. A total of 114 consecutive individuals who took the hepatitis screening test were enrolled. Twenty microliters of plasma was successfully obtained from all participants. Among the participants, two had positive results for HBs antigen and two were positive for anti-HCV; all four were > 60 years old and rarely visited the hospital. Thirty-three and 38 patients chronically infected with HBV and HCV, respectively, were examined for confirmatory assays at participating hospitals. All subjects with undetectable serum levels of HBs antigen and anti-HCV had undetectable levels of both markers in fingertip blood, and the levels in serum and fingertip blood were significantly correlated (p<0.01). The lower detection limit of HBs antigen was defined as 0.005 IU/ml, and the cut-off value of anti-HCV was 1.0 by using 10-μl fingertip blood samples. The fingertip blood sampling described herein may be adequate in mass examinations for hepatitis virus testing in Japan.
我们调查了在日本进行大规模肝炎病毒感染检查时，通过指尖采血采集少量血液是否足够。在笠冈市和小豆岛的健康博览会上进行了一项横断面调查，参与者在那里接受了肝炎筛查测试。共有114名连续接受肝炎筛查测试的个体被纳入研究。所有参与者均成功获得了20微升血浆。在参与者中，有2人HBs抗原检测呈阳性，2人抗-HCV检测呈阳性；这4人年龄均超过60岁，很少去医院就诊。分别有33例和38例慢性HBV和HCV感染者在参与研究的医院接受了确证检测。所有血清中HBs抗原和抗-HCV检测不到的受试者，其指尖血中这两种标志物的水平也检测不到，且血清和指尖血中的水平显著相关（p<0.01）。使用10微升指尖血样本时，HBs抗原的检测下限定义为0.005 IU/ml，抗-HCV的临界值为1.0。本文所述的指尖采血在日本大规模肝炎病毒检测检查中可能是足够的。
