College of Life Sciences, Qufu Normal University, Qufu, 273165, Shandong, People's Republic of China.
Taishan Academy of Forestry Sciences, Tai'an, 271000, Shandong, People's Republic of China.
Environ Sci Pollut Res Int. 2018 Sep;25(25):24798-24806. doi: 10.1007/s11356-018-2536-7. Epub 2018 Jun 21.
Harmful algal blooms (HABs) induced by Prorocentrum donghaiense occur frequently and cause a serious threat to the marine ecosystem. In this study, antialgal effects of α-linolenic acid (ALA) that is generally extracted from diverse macroalga on P. donghaiense were investigated. Specifically, the growth, cellular morphology and ultrastructure, reactive oxygen species (ROS) content, mitochondrial membrane potential (MMP), cytochrome C (Cyt-C), and caspase-9,3 activity of untreated and treated P. donghaiense were investigated. The results showed that ALA significantly inhibited the growth of P. donghaiense. Under ALA exposure, the cellular morphology and ultrastructure were damaged. ALA also induced ROS overproduction in the algal cells, decreased MMP, induced Cyt-C release, and activated caspase-9,3, which strongly relates to algal apoptosis. In summary, this study revealed the responses of morphology and physiology of P. donghaiense when exposed under ALA, and shows the potential of biotechnology on controlling P. donghaiense.
东海原甲藻（Prorocentrum donghaiense）引发的有害藻华（HABs）频繁发生，对海洋生态系统构成严重威胁。本研究调查了从各种大型藻类中普遍提取的α-亚麻酸（ALA）对东海原甲藻的抑藻作用。具体而言，研究了未经处理和经处理的东海原甲藻的生长、细胞形态和超微结构、活性氧（ROS）含量、线粒体膜电位（MMP）、细胞色素 C（Cyt-C）和半胱天冬酶-9、3 活性。结果表明，ALA 显著抑制了东海原甲藻的生长。在 ALA 暴露下，细胞形态和超微结构受损。ALA 还诱导藻类细胞中 ROS 的过度产生，降低 MMP，诱导 Cyt-C 释放，并激活 caspase-9、3，这与藻类细胞凋亡密切相关。总之，本研究揭示了东海原甲藻在 ALA 暴露下形态和生理的反应，并展示了生物技术在控制东海原甲藻方面的潜力。
