Kitajima Ken
Bioscience and Biotechnology Center, Nagoya University, Nagoya, Japan.
Methods Mol Biol. 2018;1804:429-435. doi: 10.1007/978-1-4939-8552-4_22.
KDN is an abbreviated name of 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid, and belongs to sialic acid members like N-acetylneuraminic acid (Neu5Ac), and N-glycolylneuraminic acid (Neu5Gc). The aminoacyl group at C5 position of Neu5Ac is replaced by a hydroxyl group in KDN. Like Neu5Ac, KDN exists in various glycoconjugates including glycosphingolipids in vertebrates and gram-negative bacteria. Because of its unique properties, some methods are specifically applicable to KDN residue, although most detection methods for Neu5Ac are also applicable. In this chapter, methods for identification of KDN residues in glycosphingolipids are described, focusing on two methods that are often used, i.e., the fluorescent HPLC analysis and the TLC immunostaining with the antibodies specific to α2,3- and α2,8-KDN residues.
KDN是2-酮-3-脱氧-D-甘油-D-半乳糖壬糖酸的缩写名称，属于唾液酸成员，如N-乙酰神经氨酸（Neu5Ac）和N-糖基神经氨酸（Neu5Gc）。在KDN中，Neu5Ac C5位的氨酰基被一个羟基取代。与Neu5Ac一样，KDN存在于各种糖缀合物中，包括脊椎动物和革兰氏阴性细菌中的糖鞘脂。由于其独特的性质，尽管大多数Neu5Ac检测方法也适用于KDN，但有些方法专门适用于KDN残基。在本章中，将描述糖鞘脂中KDN残基的鉴定方法，重点介绍两种常用方法，即荧光HPLC分析和用针对α2,3-和α2,8-KDN残基的特异性抗体进行TLC免疫染色。
