Qu Xue-Song, Shi Hui, Cao Xiu-Li, Dong Xue-Fan, Li Tian, Jiao Juan-Juan, Qi Jin-Shun, Wu Mei-Na
Department of Physiology, Shanxi Medical University, Key Laboratory for Cellular Physiology of Ministry of Education, Taiyuan 030001.
Intensive Care Unit, Chifeng Municipal Hospital of Inner Mongolia, Chifeng 024000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 May 8;33(5):405-409. doi: 10.12047/j.cjap.5498.2017.098.
To investigate the effects of adiponectin (APN) on anxiety and memory impairment of 9-month-old triple transgenic Alzheimer's disease (3xTg-AD) model mice.
The 9-month-old 3xTg-AD mice and C57BL/6J mice were randomly divided into four groups (=8 for each group):Wild type(WT)+Saline, 3xTg-AD +Saline, WT+APN and 3xTg-AD +APN group. All mice were implanted cannula in lateral ventricle and each mouse was intracerebroventricular injected with adiponectin or saline under free moving condition after 7 days recovery. The anxiety and memory ability of each mouse were observed by using open field test, object recognition task and Y-maze test.
①In the open field test, compared to WT+Saline group, the time of 3xTg-AD +Saline mice spent in center was significantly decreased, and the time spent in periphery was obviously increased. However, APN treatment effectively reversed the phenomenon appeared in 3xTg-AD mice, indicating that APN could alleviate the anxiety observed in 3xTg-AD mice. ②In novel object recognition task, the discrimination index of 3xTg-AD+Saline group was (-16.7±10.1)%, significantly lower than (18.0±8.2)% in WT+Saline group (<0.01) and (15.7±8.8)% in 3xTg-AD+APN group (<0.01), which indicated that APN could effectively prevent the recognition memory impairment in 3xTg mice. ③In Y-maze test, the spontaneous alternation rate of 3xTg-AD +Saline group was (40.0±1.7)%,significantly lower than (56.6±4.6)% in WT+Saline group and (53.9±5.6)% in 3xTg-AD +APN group (<0.01), which indicated that APN could prevent working memory impairment in 3xTg-AD mice.
Adiponectin could effectively alleviate the anxiety and reverse the impairment of recognition memory and working memory of 9-month-old 3xTg-AD mice, and might play an important role in the prevention and treatment of AD.
探讨脂联素（APN）对9月龄三转基因阿尔茨海默病（3xTg-AD）模型小鼠焦虑及记忆障碍的影响。
将9月龄的3xTg-AD小鼠和C57BL/6J小鼠随机分为四组（每组n = 8）：野生型（WT）+生理盐水组、3xTg-AD +生理盐水组、WT+APN组和3xTg-AD +APN组。所有小鼠均在侧脑室植入套管，恢复7天后，在自由活动状态下对每只小鼠进行脑室内注射脂联素或生理盐水。采用旷场试验、物体识别任务和Y迷宫试验观察每只小鼠的焦虑和记忆能力。
①在旷场试验中,与WT+生理盐水组相比，3xTg-AD +生理盐水组小鼠在中央区域停留的时间显著减少，而在外围区域停留的时间明显增加。然而，APN治疗有效地逆转了3xTg-AD小鼠出现的这种现象，表明APN可以减轻3xTg-AD小鼠的焦虑。②在新物体识别任务中，3xTg-AD+生理盐水组的辨别指数为（-16.7±10.1）%，显著低于WT+生理盐水组的（18.0±8.2）%（P<0.01）和3xTg-AD+APN组的（15.7±8.8）%（P<0.01），这表明APN可以有效预防3xTg小鼠的识别记忆障碍。③在Y迷宫试验中，3xTg-AD +生理盐水组的自发交替率为（40.0±1.7）%，显著低于WT+生理盐水组的（56.6±4.6）%和3xTg-AD +APN组的（53.9±5.6）%（P<0.01），这表明APN可以预防3xTg-AD小鼠的工作记忆障碍。
脂联素可有效减轻9月龄3xTg-AD小鼠的焦虑，并逆转其识别记忆和工作记忆障碍，可能在AD的防治中发挥重要作用。
