Lin Xiao-Jing, Yang Hong-Fang, Wang Xiao-Hui
School of Kinesiology, Shanghai University of Sport, Shanghai 200438, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 May 8;33(5):426-430. doi: 10.12047/j.cjap.5495.2017.103.
To investigate the effects of aerobic exercise and dieting on chemerin and its receptor chemokine like receptor-1 (CMKLR1) in the livers of type 2 diabetes mellitus (DM) rats and its possible roles on the improvements of blood glucose and lipid metabolism.
Fifty male SD rats were randomly divided into control group (Con, =6) fed with normal diet and DM model group (=44) fed with high fat diet. After 8-week feeding, DM model rats were intraperitoneal injected streptozocin (STZ) at the dose of 30 mg/kg body weight to establish DM rats. The successfully established diabetes rats were randomly divided into 4 groups (=6):diabetes control group (DM), diabetes exercise group (EDM), diabetes dieting group by feeding normal diet (NDM) and diabetes exercise plus dieting group (ENDM). During the following 4 weeks, exercise rats participated in moderate intensity aerobic exercise on a treadmill with a progressing increasing load for 4 weeks. Fasting blood glucose (FBG) was determined by Roche glucometer. Serum levels of triglyceride (TG), total cholesterol (TC) and low-density lipoprotein (LDL)) were detected by using full automatic biochemical analyzer. The mRNA and protein expressions of chemerin and CMKLR1 in the livers were detected by real time PCR and Western blot, respectively.
Accompanied with the increased serum levels of TC, TG, LDL and FBG in DM rats, the mRNA and protein levels of chemerinand CMKLR1 in the livers of DM rats were significant up-regulated. The serum levels of TC, TG, LDL and FBG were reduced in EDM, NDM and ENDM rats compared with those of DM rats, meanwhile,decreased protein levels of chemerin were found in the livers of EDM, NDM and ENDM rats with most significant in ENDM rats while increased protein levels of CMKLR1 in the livers of NDM and ENDM rats.
Four weeks of aerobic exercise with or without dieting decreased chemerin and increased CMKLR1 in the livers of DM rats at the protein levels, which might be attributed to the improvement of blood glucose and lipid metabolism of DM rats.
探讨有氧运动和节食对2型糖尿病（DM）大鼠肝脏中chemerin及其受体趋化因子样受体1（CMKLR1）的影响，以及其对改善血糖和脂质代谢的可能作用。
将50只雄性SD大鼠随机分为正常饮食的对照组（Con，n = 6）和高脂饮食的DM模型组（n = 44）。喂养8周后，对DM模型大鼠腹腔注射剂量为30 mg/kg体重的链脲佐菌素（STZ）以建立DM大鼠。将成功建立的糖尿病大鼠随机分为4组（n = 6）：糖尿病对照组（DM）、糖尿病运动组（EDM）、通过喂食正常饮食的糖尿病节食组（NDM）和糖尿病运动加节食组（ENDM）。在接下来的4周内，运动组大鼠在跑步机上进行中等强度的有氧运动，负荷逐渐增加，持续4周。采用罗氏血糖仪测定空腹血糖（FBG）。使用全自动生化分析仪检测血清甘油三酯（TG）、总胆固醇（TC）和低密度脂蛋白（LDL）水平。分别通过实时PCR和蛋白质免疫印迹法检测肝脏中chemerin和CMKLR1的mRNA和蛋白质表达。
随着DM大鼠血清TC、TG、LDL和FBG水平的升高，DM大鼠肝脏中chemerin和CMKLR1的mRNA和蛋白质水平显著上调。与DM大鼠相比，EDM、NDM和ENDM大鼠的血清TC、TG、LDL和FBG水平降低，同时，EDM、NDM和ENDM大鼠肝脏中chemerin的蛋白质水平降低，其中ENDM大鼠最为显著，而NDM和ENDM大鼠肝脏中CMKLR1的蛋白质水平升高。
四周的有氧运动无论是否节食均可使DM大鼠肝脏中chemerin蛋白水平降低，CMKLR1蛋白水平升高，这可能归因于DM大鼠血糖和脂质代谢的改善。
