[Establishment of neuritis migration model induced by Slit 2N and silk fibroin mixture in the rat hippocampal neurons ].

OBJECTIVE

To explore a simply feasible and affordable method to establish neuritis migration model induced by Slit 2N and silk fibroin mixture in the rat hippocampal neurons .

METHODS

Neurons were derived from SD rat hippocampal tissues and cultured with a special cell culture-plate . The cultured neurons were divided into four groups, named as control group, pure silk fibroin, pure Slit 2N and Slit 2N mixture with silk fibroin (mixture group), and 50 different neurons were randomly selected in each group. Moreover, we photographed and recorded the soma coordinate and added the silk fibroin, Slit 2N and mixture to each neurite with a distance of 100 μm, except control group. Record again after 30 min. Property and positive rate of cells were identified by immunofluorescence staining.

RESULTS

Neurites of the pure Slit 2N group and the mixture group became shorter, and there was no significant change in the pure silk fibroin and control groups. The results showed that the average duration and length difference before and after changed were in descending order of mixture group, Slit 2N group, silk fibroin group (<0.05), and the silk fibroin and control groups were no significant change (>0.05). The positive rate of MAP-2 in four groups was more than 90%.

CONCLUSIONS

There were no significant effects of Silk fibroin on induced by Slit 2N in rat hippocampal neuron migration. It had an effect on reducing Slit 2N diffusion rate and extending its working time. It provides an advantageous construction method on based 3D directed neural repair for the treatment of central nervous system diseases.