Liu Cai-Li, Wang Ying-Ying
Department of Clinical Laboratory the Maternal and Child Health Hospital of Anyang City, Anyang 455000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 Apr 8;33(4):373-376. doi: 10.12047/j.cjap.5569.2017.090.
To investigate the effects of etanercept on collagen induced arthritis rats.
The rheumatoid arthritis model was established subcutaneous injection of bovine type Ⅱ collagen, and the effects of etanercept was compared through three groups:①the normal control;②arthritis model treated with saline;③arthritis model treated with etanercept (0.5 mg/kg, intraperitoneal injection) with 10 rats in each group;group②and group③ were selected by arthritis index (≥ 2)and grouped randomly. The body weight and toe volume were measured at just before and during treatment weekly. Rats were sacrificed after 4 weeks of treatment, and the serum levels of tumor necrosis factor α(TNF-α), angiopoietin-1(Ang-1), IL-1β, IL-6 and IL-8 were tested by ELISA. Nuclear factor-kappa B(NF-κB) levels and subcellular locations in toe muscles were test by Western blot.
Etanercept significantly relieved inflammation of rheumatoid arthritis, lowered the TNF-α and relative inflammatory factors levels compared with saline treatment (<0.05). The results of Western blot showed that etanercept significantly suppressed the nuclear location of NF-κB p105/p50 caused by inflammation.
The anti-inflammatation effect of etanercept may be related to its activity in inhibiting the activation of TNF-α/NF-κB pathway.
研究依那西普对胶原诱导性关节炎大鼠的影响。
通过皮下注射牛Ⅱ型胶原建立类风湿性关节炎模型，将大鼠分为三组比较依那西普的作用：①正常对照组；②用生理盐水治疗的关节炎模型组；③用依那西普（0.5mg/kg，腹腔注射）治疗的关节炎模型组，每组10只大鼠；②组和③组按关节炎指数（≥2）筛选并随机分组。每周在治疗前及治疗期间测量大鼠体重和足趾体积。治疗4周后处死大鼠，采用酶联免疫吸附测定法检测血清肿瘤坏死因子α（TNF-α）、血管生成素-1（Ang-1）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）水平。采用蛋白质免疫印迹法检测足趾肌肉中核因子-κB（NF-κB）水平及亚细胞定位。
与生理盐水治疗组相比，依那西普显著减轻类风湿性关节炎炎症，降低TNF-α及相关炎症因子水平（<0.05）。蛋白质免疫印迹法结果显示，依那西普显著抑制炎症引起的NF-κB p105/p50核定位。
依那西普的抗炎作用可能与其抑制TNF-α/NF-κB通路激活的活性有关。
