[细胞粘附分子CHL1缺乏对炎症性肠病的影响]
[Effects of CHL1 deficiency,a cell adhesion molecule,on the inflammatory bowel disease].
作者信息
Wang Xiao-Meng, Zhao Tong, Cheng Xiang, Guo Ning, Zhu Ling-Ling, Shi Ming, Wu Kui-Wu
机构信息
Department of Brain Science and Stress, Institute of Cognition and Brain Sciences, Beijing 100850.
Jiangsu Province Cancer Biotherapy Institute, Xuzhou 221004, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 Jan 8;34(1):4-7. doi: 10.12047/j.cjap.5567.2018.002.
OBJECTIVE
To investigate the effects of deficiency of CHL1 in inflammatory bowel disease (IBD).
METHODS
Dextran Sulfate Sodium (DSS)-induced colitis model was used to study the effects of deficiency of CHL1 on the development of IBD. Ten CHL1(+/+) mice in C57/BL6 background were randomly divided into CHL1(+/+) group and DSS-induced CHL1(+/+) group. Ten CHL1(-/-) mice in C57/BL6 background were randomly divided into CHL1(-/-) group and DSS-induced CHL1(-/-) group. DSS-induced CHL1(+/+) group and DSS-induced CHL1(-/-)group were fed with 1.5% DSS for 7 days, and then drinking distilled water for 2 days. CHL1(+/+) group and CHL1(-/-) group as control group were fed with distilled water for 9 days. The changes of weight, survival, fecal blood and the change of colon length in this study were observed.
RESULTS
On the 7 day, the weight of DSS-induced CHL1(-/-) group were reduced significantly, and DSS-induced CHL1(-/-) group had extreme mortality on the 9th day. The fecal blood of DSS-induced CHL1(-/-) group also had higher score than that of DSS-induced CHL1(+/+) group. In the DSS-induced CHL1(-/-) group,the length of colon was shortened obviously.
CONCLUSIONS
The loss of CHL1 aggravates the development of IBD.
目的
研究CHL1缺乏在炎症性肠病（IBD）中的作用。
方法
采用葡聚糖硫酸钠（DSS）诱导的结肠炎模型来研究CHL1缺乏对IBD发展的影响。将10只C57/BL6背景的CHL1(+/+)小鼠随机分为CHL1(+/+)组和DSS诱导的CHL1(+/+)组。将10只C57/BL6背景的CHL1(-/-)小鼠随机分为CHL1(-/-)组和DSS诱导的CHL1(-/-)组。DSS诱导的CHL1(+/+)组和DSS诱导的CHL1(-/-)组用1.5% DSS喂养7天，然后饮用蒸馏水2天。CHL1(+/+)组和CHL1(-/-)组作为对照组用蒸馏水喂养9天。观察本研究中体重、生存率、粪便潜血及结肠长度的变化。
结果
第7天，DSS诱导的CHL1(-/-)组体重显著降低，且DSS诱导的CHL1(-/-)组在第9天死亡率极高。DSS诱导的CHL1(-/-)组粪便潜血评分也高于DSS诱导的CHL1(+/+)组。在DSS诱导的CHL1(-/-)组中，结肠长度明显缩短。
结论
CHL1缺失会加重IBD的发展。
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