黄芪总黄酮对病毒性心肌炎小鼠心律失常及内质网应激的影响

[Effects of total flavonids of astragalus on arrhythmia,endoplasmic reticulum stress in mice with viral myocarditis].

作者信息

Liu Hao, Hurile Bate, Xiong Ying, Wei Cheng-Xi, Xuan Li-Ying, Wang Yu, Zhao Ming

机构信息

Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028000.

the First Clinical College of Inner Mongolia Medical University, Hohhot 100059.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 Jan 8;34(1):16-18. doi: 10.12047/j.cjap.5572.2018.005.

DOI:10.12047/j.cjap.5572.2018.005

PMID:29926652

Abstract

OBJECTIVE

To investigate the effects of total flavonids of astragalus(TFA) on arrhythmia, endoplasmic reticulum stress and connexcin in mice with viral myocarditis and to clarify the mechanisms of TFA against viral myocarditis complicated with arrhythmia.

METHODS

Thirty-six male Balb/c mice were randomly divided into control group, viral myocarditis group and total flavonoids group (=12). The mice of viral myocarditis were intraperitonealy injected with 0.1 ml/day 10-950 TCID CVB3 for 3 days. The mice of TFA group were intraperitoneal injected with 0.1 ml/day 10-950 TCID CVB3 for 3 days and treated with 0.1ml, 20 mg/L TFA by tail vein injection. At the end of the experiment, arrhythmia was detected by electrocardiogram, the heart of mice were stained by HE, the expressions of glucose-regulated protein 78(GRP78), endoplasmic reticulum stress signaling pathway factor activating transcription factor 4(ATF4) and connexcin 43(Cx43) were detected by Western blot.

RESULTS

The expressions of GRP78 and ATF4 were increased and the expression of Cx43 was decreased in viral myocarditis, while TFA inhibited these effect of viral myocarditis in heart of mice.

CONCLUSIONS

The antiarrhythmic effect of TFA may be related to the alleviation of endoplasmic reticulum stress and the increase of Cx43 expression.

摘要

目的

探讨黄芪总黄酮（TFA）对病毒性心肌炎小鼠心律失常、内质网应激及连接蛋白的影响，阐明TFA抗病毒性心肌炎并发心律失常的机制。

方法

将36只雄性Balb/c小鼠随机分为对照组、病毒性心肌炎组和总黄酮组（每组12只）。病毒性心肌炎组小鼠腹腔注射0.1 ml/天10 - 950 TCID的柯萨奇病毒B3（CVB3），连续3天。TFA组小鼠腹腔注射0.1 ml/天10 - 950 TCID的CVB3，连续3天，并通过尾静脉注射0.1 ml、20 mg/L的TFA进行治疗。实验结束时，通过心电图检测心律失常，用苏木精-伊红（HE）染色法对小鼠心脏进行染色，采用蛋白质免疫印迹法检测葡萄糖调节蛋白78（GRP78）、内质网应激信号通路因子激活转录因子4（ATF4）和连接蛋白43（Cx43）的表达。