Dos Santos Yago Queiroz, de Veras Bruno Oliveira, de França Anderson Felipe Jácome, Gorlach-Lira Krystyna, Velasques Jannaina, Migliolo Ludovico, Dos Santos Elizeu Antunes
Laboratório de Química e Função de Proteínas Bioativas, Universidade Federal do Rio Grande do Norte, Natal 59072-970, Brasil.
Departamento de Biologia Celular e Molecular, Universidade Federal da Paraíba, João Pessoa 58051-90, Brasil.
J Microbiol Biotechnol. 2018 Jul 28;28(7):1078-1085. doi: 10.4014/jmb.1802.02037.
A salt-tolerant cellulase secreted by a marine sp. SR22 strain with wide resistance to temperature and pH was purified and characterized. Its approximate mass was 37 kDa. The endoglucanase, named as Bc22Cel, was purified by ammonium sulfate precipitation, gel filtration chromatography, and extraction from the gel after non-reducing sodium dodecyl sufate-polyacrylamide gel electrophoresis. The optimal pH value and temperature of Bc22Cel were 6.5 and 60°C, respectively. The purified Bc22Cel showed a considerable halophilic property, being able to maintain more than 70% of residual activity even when pre-incubated with 1.5 M NaCl for 1 h. Kinetic analysis of the purified enzyme showed the and to be 0.704 mg/ml and 29.85 μmol·ml·min, respectively. Taken together, the present data indicate Bc22Cel as a potential and useful candidate for industrial applications, such as the bioconversion of sugarcane bagasse to its derivatives.
从一株对温度和pH具有广泛耐受性的海洋菌株SR22中分泌的一种耐盐纤维素酶被纯化并进行了表征。其近似分子量为37 kDa。这种内切葡聚糖酶被命名为Bc22Cel，通过硫酸铵沉淀、凝胶过滤色谱以及在非还原十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后从凝胶中提取进行纯化。Bc22Cel的最佳pH值和温度分别为6.5和60°C。纯化后的Bc22Cel表现出相当强的嗜盐特性，即使在与1.5 M NaCl预孵育1小时后仍能保持超过70%的残余活性。对纯化酶的动力学分析表明，其Km和Vmax分别为0.704 mg/ml和29.85 μmol·ml·min。综上所述，目前的数据表明Bc22Cel是工业应用（如甘蔗渣向其衍生物的生物转化）中一个有潜力且有用的候选物。
