Shanghai Jiao Tong University Affiliated 6th Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, P. R. China.
Nanoscale. 2018 Jul 9;10(26):12461-12471. doi: 10.1039/c8nr01888j.
With the increasing demands for high-throughput multiplexed bioassays, quantum dot (QD)-encoded microbeads as biocarriers for various bioreactions have attracted considerable attention. However, three key requirements for these biocarriers are still longstanding issues: a stable fluorescence intensity, a large encoding capacity and abundant surface functional groups. Here, a novel one-pot strategy is developed, generating functionalized QD-encoded microspheres with a strong fluorescence intensity and optical stability. With poly(styrene-co-maleic anhydride) (PSMA) molecules as mediators, the encapsulation of QDs and carboxylation of the bead surface are integrated together, greatly improving the preparation efficiency and guaranteeing their potential application in biodetection. Moreover, the mechanism for preparing QD-doped beads is further proposed, which helps to precisely manipulate the preparation process and accurately encode the beads. Through this approach, a single- and dual-color barcode library of QD-encoded microspheres has been successfully established, which demonstrates their great potential in suspension arrays.
随着高通量多重生物分析的需求不断增加，作为各种生物反应生物载体的量子点（QD）编码微球引起了相当大的关注。然而，这些生物载体仍然存在三个关键要求：稳定的荧光强度、大容量的编码能力和丰富的表面功能基团。在这里，开发了一种新的一锅法策略，生成具有强荧光强度和光学稳定性的功能化 QD 编码微球。聚（苯乙烯-co-马来酸酐）（PSMA）分子作为媒介，将 QD 的封装和珠表面的羧基化集成在一起，大大提高了制备效率，并保证了它们在生物检测中的潜在应用。此外，还提出了制备 QD 掺杂珠的机理，有助于精确控制制备过程并准确编码珠。通过这种方法，成功建立了单和双色 QD 编码微球的条码文库，展示了它们在悬浮阵列中的巨大潜力。
