School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, People's Republic of China.
Aerospace Composites Research Institute, Xi'an, People's Republic of China.
Environ Technol. 2020 Jan;41(1):86-99. doi: 10.1080/09593330.2018.1491636. Epub 2018 Jul 9.
Optimizing supports for microorganisms is required for bioreactors. Carbon fibres (CF) were employed as supports for microorganisms. To optimize CF supports for immobilizing bacterial cells, we used methods of nitric acid oxidation and calcium ion coverage. We evaluated the capacity of these CF supports (untreated CF, nitric acid oxidation CF and Ca-covered CF) via bacterial cell adhesion tests, based on extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory. The results implied that because of the high hamaker constants, oxidized CF supports had higher capacity in this regard than untreated CF supports. However, the growing oxygen groups increased the negative zeta potential of CF supports, thus likely to reduce their capacity, in accordance with XDLVO theory. Since the Ca coverage could decrease the negative zeta potentials of CF without reducing the hamaker constants, it could enhance the capacity of oxidized CF supports. We concluded that a combination of nitric acid oxidation and Ca coverage could increase the capacity of CF supports to immobilize bacterial cells.
生物反应器需要优化对微生物的支持。碳纤维（CF）被用作微生物的载体。为了优化用于固定细菌细胞的 CF 载体，我们使用了硝酸氧化和钙离子覆盖的方法。我们通过细菌细胞粘附试验，根据扩展的 Derjaguin-Landau-Verwey-Overbeek（XDLVO）理论，评估了这些 CF 载体（未处理的 CF、硝酸氧化的 CF 和 Ca 覆盖的 CF）的能力。结果表明，由于高 Hamaker 常数，氧化的 CF 载体在这方面比未处理的 CF 载体具有更高的容量。然而，随着含氧基团的增加，CF 载体的负 ζ 电位增加，因此可能会根据 XDLVO 理论降低其容量。由于 Ca 覆盖不会降低 Hamaker 常数而降低 CF 的负 ζ 电位，因此它可以增强氧化 CF 载体的能力。我们得出结论，硝酸氧化和 Ca 覆盖的结合可以提高 CF 载体固定细菌细胞的能力。
