Li Hao, Shi Chen-Xi, Liu Hui, Zhang Hai-Han, Sang Huai-Ming, Soyfoo Muhammad-Djaleel, Cao Jiu-Liang, Xu Shun-Fu, Jiang Jian-Xia
Department of Pathophysiology, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China.
Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
Oncol Lett. 2018 Jun;15(6):9711-9718. doi: 10.3892/ol.2018.8585. Epub 2018 Apr 26.
Piwi like RNA-mediated gene silencing 1 (Hiwi) is a human homolog of the Piwi gene family that has been reported to be upregulated in hepatocellular carcinoma (HCC). The present study aimed to investigate the role of Hiwi in the initiation and development of HCC and . Adenovirus-mediated Hiwi overexpression was established in primary murine hepatocytes and SMMC7721 HCC cells. Cell viability and proliferation were assessed using MTT and EdU assays, respectively. Cell migration was measured using a scratch migration assay. The cell cycle was assessed using flow cytometry, and the expression of genes associated with the epithelial mesenchymal transition (EMT) was assessed using reverse transcription-quantitative polymerase chain reaction. SMMC7721 cells that stably express Hiwi were also generated and injected subcutaneously into the nude mice, and tumor growth was examined. Recombinant adenovirus encoding green fluorescent protein or Hiwi was delivered by injection into the tail vein, and its effect on murine hepatocyte gene expression was studied. The present study revealed that the overexpression of Hiwi did not affect the proliferation or migration of liver cancer cells and failed to suppress perifosine- or doxorubicin-induced apoptosis . The tumors of mice that were injected with Hiwi-expressing SMMC7721 cells were not significantly larger compared with mice that were injected with control SMMC7721 cells. Hiwi overexpression did not noticeably alter the expression of genes involved in EMT, either or . The results of the present study indicate that although expression of Hiwi is associated with HCC development and progression in the clinic, it does not act as an oncogene in liver cancer cells.
PIWI样RNA介导的基因沉默1（Hiwi）是Piwi基因家族的人类同源物，据报道在肝细胞癌（HCC）中上调。本研究旨在探讨Hiwi在HCC发生发展中的作用。在原代小鼠肝细胞和SMMC7721 HCC细胞中建立了腺病毒介导的Hiwi过表达。分别使用MTT和EdU试验评估细胞活力和增殖。使用划痕迁移试验测量细胞迁移。使用流式细胞术评估细胞周期，并使用逆转录定量聚合酶链反应评估与上皮-间质转化（EMT）相关的基因表达。还生成了稳定表达Hiwi的SMMC7721细胞并皮下注射到裸鼠中，检查肿瘤生长情况。通过尾静脉注射递送编码绿色荧光蛋白或Hiwi的重组腺病毒，并研究其对小鼠肝细胞基因表达的影响。本研究表明，Hiwi的过表达不影响肝癌细胞的增殖或迁移，也不能抑制哌立福新或阿霉素诱导的细胞凋亡。与注射对照SMMC7721细胞的小鼠相比，注射表达Hiwi的SMMC7721细胞的小鼠肿瘤没有明显更大。Hiwi的过表达也没有显著改变参与EMT的基因表达。本研究结果表明，尽管Hiwi的表达在临床上与HCC的发生发展相关，但它在肝癌细胞中并不作为癌基因起作用。
