Wang Ning, Zhang Zhenni, Lv Jianrui
Department of Anesthesiology, Second Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an, Shaanxi 710002, P.R. China.
Oncol Lett. 2018 Jul;16(1):459-466. doi: 10.3892/ol.2018.8616. Epub 2018 May 2.
Fentanyl is one of the most commonly used intravenous anesthetic agents during cancer resection surgery, but the effect of fentanyl on esophageal squamous cell carcinoma (ESCC) remains unclear. The aim of the present study was to investigate the involvement of microRNA 302b (miR-302b) in the anti-proliferation and anti-invasion effects of fentanyl in ESCC. In the present study, the effects of fentanyl on cell proliferation, apoptosis and invasion were detected using MTT assays, flow cytometry and Transwell assays in ESCC Eca109 and TE1 cell lines. Subsequently, expression of miR-302b was determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). RT-qPCR and western blot analysis were performed in order to evaluate the expression of ErbB4, a target of miR-302b. Furthermore, anti-miR were used to inhibit miR-302b in fentanyl-treated ESCC cells in order to evaluate the role of miR-302b in the effect of fentanyl on malignant behaviors. Fentanyl inhibited the proliferation of Eca109 and TE1 cells in a dose- and time-dependent manner. Following exposure to fentanyl for 48 h, Eca109 and TE1 cells exhibited increased apoptosis and decreased invasion. Furthermore, fentanyl upregulated miR-302b expression, but downregulated ErbB4 expression. Finally, loss of miR-302b using the anti-miR technique reversed the effect of fentanyl on cell proliferation, apoptosis and invasion in the two ESCC cell lines. Taken together, the results of the present study indicated that fentanyl inhibits the proliferation and invasion of ESCC cells through upregulation of miR-302b.
芬太尼是癌症切除手术中最常用的静脉麻醉剂之一，但芬太尼对食管鳞状细胞癌（ESCC）的影响仍不清楚。本研究的目的是探讨微小RNA 302b（miR-302b）在芬太尼对ESCC的抗增殖和抗侵袭作用中的作用。在本研究中，使用MTT法、流式细胞术和Transwell法检测芬太尼对ESCC Eca109和TE1细胞系细胞增殖、凋亡和侵袭的影响。随后，使用逆转录定量聚合酶链反应（RT-qPCR）测定miR-302b的表达。进行RT-qPCR和蛋白质印迹分析以评估miR-302b的靶标ErbB4的表达。此外，使用抗miR抑制芬太尼处理的ESCC细胞中的miR-302b，以评估miR-302b在芬太尼对恶性行为影响中的作用。芬太尼以剂量和时间依赖性方式抑制Eca109和TE1细胞的增殖。暴露于芬太尼48小时后，Eca109和TE1细胞凋亡增加，侵袭减少。此外，芬太尼上调miR-302b表达，但下调ErbB4表达。最后，使用抗miR技术使miR-302b缺失逆转了芬太尼对两种ESCC细胞系细胞增殖、凋亡和侵袭的影响。综上所述，本研究结果表明芬太尼通过上调miR-302b抑制ESCC细胞的增殖和侵袭。
