鲤鱼（鲤科鲤属）红细胞中钙蛋白酶II（高钙需求型钙蛋白酶）的检测及其某些特性
Detection and some properties of calpain II (high-Ca2+-requiring form of calpain) in carp (Cyprinus carpio) erythrocytes.
作者信息
Toyohara H, Makinodan Y, Ikeda S
出版信息
Comp Biochem Physiol B. 1985;81(3):583-6. doi: 10.1016/0305-0491(85)90370-0.
In order to examine the existence of calpain I, a low (micromolar)-Ca2+-requiring form of calpain, in fish tissues, carp erythrocytes were chosen as the experimental material, since only calpain I is known to exist in mammalian erythrocytes. By DEAE-cellulose chromatography, calpain and calpastatin (specific inhibitor for calpain) were separated from carp erythrocyte hemolysate. Carp erythrocyte calpain is classified as calpain II, a high (millimolar)-Ca2+-requiring form of calpain, from the result of Ca2+-requirement for the activity.
为了检测鱼类组织中钙蛋白酶I（一种对钙离子需求较低（微摩尔级）的钙蛋白酶形式）的存在情况，选择鲤鱼红细胞作为实验材料，因为已知哺乳动物红细胞中仅存在钙蛋白酶I。通过DEAE-纤维素色谱法，从鲤鱼红细胞溶血产物中分离出钙蛋白酶和钙蛋白酶抑制蛋白（钙蛋白酶的特异性抑制剂）。根据活性对钙离子的需求结果，鲤鱼红细胞钙蛋白酶被归类为钙蛋白酶II，即一种对钙离子需求较高（毫摩尔级）的钙蛋白酶形式。
Zotero 插件