Department of Urology, Roswell Park Comprehensive Cancer Center, Elm and Carlton Streets, Buffalo, NY, 14263, USA.
Department of Cancer Prevention and Control, Roswell Park Comprehensive Cancer Center, Elm and Carlton Streets, Buffalo, NY, 14263, USA.
Mol Cell Endocrinol. 2018 Dec 5;477:121-131. doi: 10.1016/j.mce.2018.06.009. Epub 2018 Jun 18.
Steroid hormones play important roles in normal physiological functions and diseases. Sex steroids hormones are important in the biology and treatment of sex hormone-related cancer such as prostate cancer and breast cancer. Cells may take up steroids using multiple mechanisms. The conventionally accepted hypothesis that steroids cross cell membrane through passive diffusion has not been tested rigorously. Experimental data suggested that cells may take up sex steroid using an active uptake mechanism. H-testosterone uptake by prostate cancer cells showed typical transporter-mediated uptake kinetic. Cells retained testosterone taken up from the medium. The uptake of testosterone was selective for certain steroid hormones but not others. Data also indicated that the active and selective uptake mechanism resided in cholesterol-rich membrane domains, and may involve ATP and membrane transporters. In summary, the present study provided strong evidence to support the existence of an active and selective molecular mechanism for sex steroid uptake.
甾体激素在正常生理功能和疾病中发挥着重要作用。性激素在与性激素相关的癌症（如前列腺癌和乳腺癌）的生物学和治疗中非常重要。细胞可能通过多种机制摄取甾体激素。传统上认为甾体激素通过被动扩散穿过细胞膜的假设尚未得到严格检验。实验数据表明，细胞可能通过主动摄取机制摄取性激素。H-睾酮被前列腺癌细胞摄取显示出典型的转运体介导的摄取动力学。细胞保留从培养基中摄取的睾酮。睾酮的摄取对某些甾体激素具有选择性，但对其他甾体激素则没有。数据还表明，主动和选择性摄取机制存在于富含胆固醇的膜区域中，并且可能涉及 ATP 和膜转运体。总之，本研究提供了强有力的证据支持性激素摄取存在一种主动和选择性的分子机制。
