Laboratório de Bionanotecnologia, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo, 12244000, Brazil; Laboratório de Nanossensores, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo, 12244000, Brazil.
Laboratório de Nanossensores, Instituto de Pesquisa & Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo, 12244000, Brazil.
Photodiagnosis Photodyn Ther. 2018 Sep;23:212-217. doi: 10.1016/j.pdpdt.2018.06.011. Epub 2018 Jun 19.
Breast cancer is the most commonly diagnosed cancer and the second leading cause of death related to cancer among women worldwide. Screening and advancements in treatment have improved survival rate of women suffering from this ailment. Novel therapeutic techniques may further reduce cancer related mortality. One of several emerging therapeutic options is Photodynamic Therapy (PDT) that uses light activated photosensitizer (PS) inducing cell death by apoptosis and/or necrosis. Nanotechnology has made contribution to improve photosensitizer for PDT, increasing the efficiency of therapy using gold and silver nanoparticles. Efforts have been done to develop better mechanism to improve PS and consequently PDT effects. In this study, we investigate the efficacy of the PDT using gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) when mixed to methylene blue (MB) in the treatment of the human breast adenocarcinoma cell line (MDA-MB-468). The MDA-MB-468 was treated in the presence of different MB concentrations with/without AuNPs or AgNPs. The colloidal solution of AgNPs showed a plasmon resonance band at 411 nm in UV-visible range and a bimodal size distribution. The results of viability analysis showed that cells treated with nanoparticles exhibited higher cytotoxicity than cells treated with only MB, improving the efficiency of the treatment in the tumor cells. The cytotoxicity effect of MB associated with AgNPs on MDA-MB-468 cell line could be related to increased reactive oxygen species production due to the release of Ag ions from nanoparticles surface, suggesting that the association between FS and AgNPs has potential as a PDT agent.
乳腺癌是全世界女性中最常见的癌症，也是癌症相关死亡的第二大主要原因。筛查和治疗的进步提高了患有这种疾病的女性的生存率。新的治疗技术可能会进一步降低癌症相关死亡率。几种新兴治疗选择之一是光动力疗法 (PDT)，它使用光激活的光敏剂 (PS) 通过细胞凋亡和/或坏死诱导细胞死亡。纳米技术为 PDT 改善光敏剂做出了贡献，使用金和银纳米粒子提高了治疗效率。已经做出了努力来开发更好的机制来改善 PS，从而提高 PDT 的效果。在这项研究中，我们研究了金纳米粒子 (AuNPs) 和银纳米粒子 (AgNPs) 与亚甲蓝 (MB) 混合用于治疗人乳腺癌腺癌细胞系 (MDA-MB-468) 的 PDT 效果。MDA-MB-468 在不同 MB 浓度存在下用/不用 AuNPs 或 AgNPs 处理。AgNPs 的胶体溶液在紫外-可见范围内显示出 411nm 的等离子体共振带和双峰尺寸分布。细胞活力分析的结果表明，用纳米粒子处理的细胞比仅用 MB 处理的细胞表现出更高的细胞毒性，从而提高了肿瘤细胞治疗的效率。MB 与 AgNPs 对 MDA-MB-468 细胞系的细胞毒性作用可能与 Ag 离子从纳米粒子表面释放导致的活性氧产生增加有关，这表明 FS 与 AgNPs 的结合具有作为 PDT 剂的潜力。
