Department of Biomedical Engineering, Catholic University of Daegu, Gyeongsan 712-702, Republic of Korea.
Department of Biomedical Engineering, Catholic University of Daegu, Gyeongsan 712-702, Republic of Korea.
J Photochem Photobiol B. 2015 May;146:34-43. doi: 10.1016/j.jphotobiol.2015.02.022. Epub 2015 Mar 9.
Although methylene blue (MB) is the most inexpensive photosensitizer with promising applications in the photodynamic therapy (PDT) for its high quantum yield of singlet oxygen generation, the clinical use of MB has been limited by its rapid enzymatic reduction in the biological environment. To enhance PDT efficacy of MB by preventing the enzymatic reduction, we have developed a new mineralization method to produce highly biocompatible MB-loaded calcium phosphate (CaP-MB) nanoparticles in the presence of polymer templates. The resulting CaP-MB nanoparticles exhibited spherical shape with a size of under 50 nm. Fourier transform infrared (FT-IR) and zeta-potential analyses confirmed the insertion of MB into the CaP-MB nanoparticles. The encapsulation of MB in CaP nanoparticles could effectively protect MB from the enzymatic reduction. In addition, the CaP-MB nanoparticles exhibited a good biocompatibility in the dark condition and significantly enhanced PDT efficacy due to apoptotic cell death against human breast cancer cells as compared with free MB, implying that CaP-MB nanoparticle system might be potentially applicable in PDT.
尽管亚甲蓝 (MB) 是最廉价的光敏剂，因其具有生成单线态氧的高量子产率而在光动力疗法 (PDT) 中有广阔的应用前景，但由于其在生物环境中被酶迅速还原，MB 的临床应用受到限制。为了通过阻止酶还原来增强 MB 的 PDT 疗效，我们开发了一种新的矿化方法，在聚合物模板的存在下生产高度生物相容的载 MB 磷酸钙 (CaP-MB) 纳米颗粒。所得的 CaP-MB 纳米颗粒呈球形，尺寸小于 50nm。傅里叶变换红外 (FT-IR) 和 ζ 电位分析证实了 MB 插入到 CaP-MB 纳米颗粒中。MB 被包封在 CaP 纳米颗粒中可以有效地保护 MB 免受酶还原。此外，与游离 MB 相比，CaP-MB 纳米颗粒在黑暗条件下具有良好的生物相容性，并显著增强了 PDT 疗效，导致人乳腺癌细胞发生凋亡性细胞死亡，这表明 CaP-MB 纳米颗粒系统可能在 PDT 中有潜在的应用。
