São Leopoldo Mandic Institute and Dental Research Center, Rua José Rocha Junqueira 13, Bairro Swift, Campinas, CEP: 13045-755, São Paulo, Brazil.
Arch Oral Biol. 2018 Oct;94:16-26. doi: 10.1016/j.archoralbio.2018.06.012. Epub 2018 Jun 11.
Investigate the long-term effect of dentin pretreatment with 0.05 or 0.1% caffeic acid phenethyl ester (CAPE) on (1) bond strength of resin composite to dentin by a three-step etch-and-rinse (Adper Scotchbond Multipurpose/ ASB) or a two-step self-etch adhesive system (Clearfil SE Bond/ CSE), (2) their fracture mode, (3) the micromorphological features of the hybrid layer formed; and (4) the level of MMP-2 in dentin (after application, using a correlative immunoexpression/quantification approach).
Composite resin blocks were fabricated on 48 third molars (n = 6), according to the type of adhesive and treatment (control, CAPE 0.05% and CAPE 0.1%). Slices were obtained for scanning electron microscopy (SEM) evaluation, and sticks were fabricated for microtensile tests (24 h and 1 year). Aliquots of dentin powder were distributed (n = 12) according to the treatment and the MMP-2 concentration was determined by ELISA.
Tukey test showed that ASB groups presented higher BS in 24 h than CSE groups. ASB presented a reduction in BS values after 1-year. ASB and CSE presented no significant differences in BS after 1-year. CAPE had no effect on BS for both adhesive systems. The predominant failure mode for the ASB groups were adhesive; when 0.1% CAPE was applied there was a predominance of mixed fractures. Regarding the CSE group, 0.05% CAPE led to more adhesive failures, and the 0.1% concentration resulted in a higher number of cohesive failures in dentin. Higher MMP-2 concentrations were detected for the groups that did not undergo demineralization treatment, and the lowest values for the ASB groups treated with CAPE. SEM analysis showed no influence of pretreatment with CAPE.
CAPE did not influence the BS of the adhesives tested, or the micromorphology of the hybrid layer, irrespective of concentration or storage time. CAPE affected the fracture pattern at 24 h, depending on the concentration and the adhesive system used. Immunoassay analysis showed that CAPE 0.1% reduced the MMP-2 concentration in the ASB adhesive without affecting bond strength to dentin.
研究 0.05%或 0.1%桂皮酸苯乙酯（CAPE）对牙本质预处理对（1）三步酸蚀-冲洗（Adper Scotchbond Multipurpose/ASB）或两步自酸蚀粘接剂系统（Clearfil SE Bond/CSE）复合树脂与牙本质粘结强度的影响，（2）其断裂模式，（3）形成的混合层的微观形态特征；以及（4）牙本质中 MMP-2 的水平（应用后，采用相关免疫表达/定量方法）。
根据粘接剂和处理方式（对照、CAPE 0.05%和 CAPE 0.1%），将 48 颗第三磨牙制成 48 个复合树脂块（n=6）。对扫描电镜（SEM）评估的切片进行了评估，并制备了微拉伸试验的棒（24 小时和 1 年）。根据处理方式将牙本质粉末等分（n=12），并通过 ELISA 测定 MMP-2 浓度。
Tukey 检验显示，ASB 组在 24 小时时的 BS 值高于 CSE 组。ASB 组在 1 年后 BS 值降低。ASB 和 CSE 组在 1 年后的 BS 无显著差异。两种粘接系统的 CAPE 对 BS 均无影响。ASB 组的主要失效模式为粘结；当应用 0.1% CAPE 时，混合断裂占主导地位。对于 CSE 组，0.05% CAPE 导致更多的粘结失败，而 0.1%浓度导致牙本质中更多的内聚性破坏。未进行脱矿处理的组检测到更高的 MMP-2 浓度，而 CAPE 处理的 ASB 组的浓度最低。SEM 分析显示 CAPE 预处理不影响粘结强度或混合层的微观形态，与浓度或储存时间无关。CAPE 在 24 小时时影响断裂模式，取决于浓度和所用的粘接系统。免疫测定分析显示，0.1% CAPE 降低了 ASB 粘接剂中的 MMP-2 浓度，而不影响牙本质的粘结强度。
