Evans C H, Ridella J D
Eur J Biochem. 1985 Aug 15;151(1):29-32. doi: 10.1111/j.1432-1033.1985.tb09064.x.
Fragments of human, rheumatoid synovium were maintained on organ culture for three days under serum-less conditions. Their conditioned media contained collagenolytic, gelatinolytic and caseinolytic activities, which were susceptible to inhibition by lanthanide ions. Of the four lanthanides tested, Sm3+ proved the best inhibitor of gelatinase and caseinase, while La3+ inhibited collagenase the most strongly. Inhibition of collagenase by La3+ was uncompetitive. A direct binding assay confirmed the greater association between collagen fibrils and collagenase in the presence of La3+. Ca2+ was not required for binding of the uninhibited enzyme to collagen, but acted to stabilize collagenase against thermoinactivation.
人类风湿性滑膜碎片在无血清条件下进行器官培养三天。其条件培养基含有胶原分解活性、明胶分解活性和酪蛋白分解活性，这些活性易受镧系离子抑制。在所测试的四种镧系元素中，Sm3+被证明是明胶酶和酪蛋白酶的最佳抑制剂，而La3+对胶原酶的抑制作用最强。La3+对胶原酶的抑制是非竞争性的。直接结合试验证实，在La3+存在的情况下，胶原纤维与胶原酶之间的结合更强。未受抑制的酶与胶原的结合不需要Ca2+，但Ca2+可使胶原酶稳定，防止热失活。
