Institute for Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
J Infect Dis. 2018 Nov 22;218(suppl_5):S355-S359. doi: 10.1093/infdis/jiy247.
The Ebola virus (EBOV) matrix protein VP40 drives budding of virions and encodes 2 overlapping late domain motifs at amino acid positions 7-13 (PTAPPEY). However, these motifs are not absolutely essential for replication in cell culture, and recently a potential third late domain motif (YPx(6)I) was proposed at amino acid positions 18-26 of VP40. To analyze the importance of this motif in viral budding, we used a transcription and replication-competent virus-like particle system. Using this system, we show that this motif does not contribute to EBOV budding or particle propagation.
埃博拉病毒（EBOV）基质蛋白 VP40 驱动病毒粒子出芽，并在氨基酸位置 7-13（PTAPPEY）编码 2 个重叠的晚期结构域基序。然而，这些基序对于细胞培养中的复制并不是绝对必需的，最近在 VP40 的氨基酸位置 18-26 提出了潜在的第三个晚期结构域基序（YPx(6)I）。为了分析该基序在病毒出芽中的重要性，我们使用了转录和复制完全的病毒样颗粒系统。使用该系统，我们表明该基序不会促进 EBOV 出芽或颗粒传播。
