Fang Wen, Li Ze, Liu Xiao-Hua, Liu Zhao-Ming, Dun Yi, Feng Hong
Tianjin University of Sport, Tianjin Key Laboratory of Physiology, Tianjin 3000381.
Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 Jun 8;33(6):544-549. doi: 10.12047/j.cjap.5601.2017.129.
To observe the changes of Prohibitin1(PHB1) contents in rat brain, heart, skeletal muscle tissue and mitochondria with acute exhaustive exercise and the effects of acute exhaustive exercise on mitochondrial function in rats, to explore the relationship among PHB1 and mitochondrial function and energy metabolism.
Acute exhaustive exercise model:The rats carried acute exhaustive exercise after 8 weeks of feeding. The heart, brain and skeletal muscle samples were collected and the mitochondria were collected to detect the changes of respiratory function and reactive oxygen species(ROS). The expression of PHB1 protein in tissues and mitochondria was detected by Western blot. The ATP content in the organs and the activity of complexes (ATP synthase activity) in mitochondria were measured by spectrophotometer.
① The ATP contents of brain, myocardium and skeletal muscle were decreased significantly after acute exhaustive exercise. ②The activities of complex V, respiratory control rates (RCR) and ROS in mitochondria of brain, myocardium and skeletal muscle were decreased significantly after acute exhaustive exercise, respiration rate state 4(ST4) was increased significantly, at the same time, respiration rate state 3(ST3) had no significant difference. ③ The expression of PHB1 in mitochondria of skeletal muscle was decreased significantly after acute exhaustive exercise, while there was no significant change in PHB1 in myocardial tissue and mitochondria. ④ The correlation analysis showed that the ATP contents in the brain, myocardium and skeletal muscle were positively correlated with the activity of complex V and the expression of PHB1 after acute exhaustive exercise.
After acute exhaustive exercise, the mitochondrial oxidative phosphorylation was reduced, the ROS production was increased, the expression of PHB1 was decreased, the ATP content and the activity of complex V were decreased in the brain and skeletal muscle of rats. Acute exhaustive exercise reduced the expression of PHB1 in mitochondria, decreased mitochondrial function, and reduced energy metabolism.
观察急性力竭运动后大鼠脑、心、骨骼肌组织及线粒体中抗增殖蛋白1（PHB1）含量的变化以及急性力竭运动对大鼠线粒体功能的影响，探讨PHB1与线粒体功能及能量代谢之间的关系。
急性力竭运动模型：喂养8周后的大鼠进行急性力竭运动。采集心脏、脑和骨骼肌样本并分离线粒体，检测呼吸功能和活性氧（ROS）的变化。采用蛋白质免疫印迹法检测组织及线粒体中PHB1蛋白的表达。用分光光度计测定器官中的ATP含量及线粒体中复合物（ATP合酶活性）的活性。
①急性力竭运动后，脑、心肌和骨骼肌的ATP含量显著降低。②急性力竭运动后，脑、心肌和骨骼肌线粒体中复合物V的活性、呼吸控制率（RCR）及ROS显著降低，呼吸速率状态4（ST4）显著升高，而呼吸速率状态3（ST3）无显著差异。③急性力竭运动后，骨骼肌线粒体中PHB1的表达显著降低，而心肌组织及线粒体中PHB1无显著变化。④相关性分析表明，急性力竭运动后，脑、心肌和骨骼肌中的ATP含量与复合物V的活性及PHB1的表达呈正相关。
急性力竭运动后，大鼠脑和骨骼肌的线粒体氧化磷酸化降低，ROS生成增加，PHB1表达降低，ATP含量及复合物V的活性下降。急性力竭运动降低了线粒体中PHB1的表达，使线粒体功能下降，能量代谢降低。
