Wang Xu-Tao, Pan Ding-Yi, Guo Wei, Chen Si-Si, Zhou Bin, Yang Jun-Jie, Qi Min-You
Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 Jun 8;33(6):564-567. doi: 10.12047/j.cjap.5601.2017.133.
To investigate the effects of ferulic acid (FA) on the streptozocin (STZ) -induced kidney injury in diabetic rats and its possible mechanisms.
Diabetes was induced in male SD rats by an injection of STZ (40 mg/kg,i.v.). After 72 hours, blood glucose levels were detected and blood glucose levels exceeded 16.7 mmol/L were diagnosed as diabetic model rats. Diabetic model rats were randomly divided into model group and FA group, ten animal in each group. Another 10 healthy male SD rats were treated as control group. The rats in FA group were treated with FA (100 mg/kg, i.g.,qd) from the 5th week since the diabetic rats model was successfully established and lasted for 8 weeks. The levels of blood glucose, body weight, organ coefficient of kidney, blood urea nitrogen and creatinine were tested. HE staining was employed to observe the pathological changes of the renal tissue. Immunohistochemistry was employed to determine the protein of nephrin and podocin.
Compared to control group, the levels of blood glucose, organ coefficient of kidney, blood urea nitrogen(BUN) and serum creatinine(sCr) were increased significantly. Renal cells from model group rats showed atrophied and disordered after HE staining and interstitial proliferation were also appeared in renal tissue of the model group. Meantime, the levels of nephrin and podocin protein were obviously decreased. These changes were significantly attenuated in the model group treated with FA.
FA can evidently ameliorate renal damage in rats with diabetic nephropathy induced by STZ, which might be related to increase the level of nephrin and podocin protein.
探讨阿魏酸（FA）对链脲佐菌素（STZ）诱导的糖尿病大鼠肾损伤的影响及其可能机制。
雄性SD大鼠通过静脉注射STZ（40mg/kg）诱导糖尿病。72小时后检测血糖水平，血糖水平超过16.7mmol/L者诊断为糖尿病模型大鼠。将糖尿病模型大鼠随机分为模型组和FA组，每组10只动物。另取10只健康雄性SD大鼠作为对照组。自糖尿病大鼠模型成功建立后第5周起，FA组大鼠给予FA（100mg/kg，灌胃，每日1次），持续8周。检测血糖、体重、肾脏器官系数、血尿素氮和肌酐水平。采用HE染色观察肾组织病理变化。采用免疫组织化学法检测nephrin和podocin蛋白。
与对照组相比，血糖、肾脏器官系数、血尿素氮（BUN）和血清肌酐（sCr）水平显著升高。模型组大鼠肾细胞经HE染色后显示萎缩和紊乱，模型组肾组织也出现间质增生。同时，nephrin和podocin蛋白水平明显降低。在FA治疗的模型组中，这些变化明显减轻。
FA可明显改善STZ诱导的糖尿病肾病大鼠的肾损伤，这可能与提高nephrin和podocin蛋白水平有关。
