基于光谱法的水溶液中免疫抑制剂药物浓度检测方法。

Optical-Spectrometry-Based Method for Immunosuppressant Medicine Level Detection in Aqueous Solutions.

机构信息

Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, 11/12 Narutowicza Street, 80-233 Gdansk, Poland.

Department of Nephrology, Transplantology and Internal Diseases, Faculty of Medicine, Medical University of Gdańsk, 3a Marii Skłodowskiej-Curie Street, 80-210 Gdansk, Poland.

出版信息

Sensors (Basel). 2018 Jun 22;18(7):2001. doi: 10.3390/s18072001.

DOI:10.3390/s18072001

PMID:29932121

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6068911/

Abstract

In this paper, an investigation into detecting immunosuppressive medicine in aqueous solutions using a spectrometry-based technique is described. Using optical transmissive spectrometry, absorbance measurements in the spectra range from 250 nm to 1000 nm were carried out for different cyclosporine A (CsA) concentrations in aqueous solutions. The experiment was conducted for samples both with and without interferent substances—glucose and sodium chloride. Using a dedicated algorithm, the measured data was analyzed and a high correlation coefficient R² = 0.8647 was achieved. The experiment showed that the described technique allowed for the detection of various CsA concentration levels in a selective, label-free and simple way. This method could be used in medicine, veterinary medicine and laboratory diagnostics.

摘要

本文描述了一种基于光谱技术检测水溶液中免疫抑制药物的方法。采用光透射光谱法，在 250nm 至 1000nm 的光谱范围内，对不同浓度的环孢素 A（CsA）在水溶液中的吸收进行了测量。实验分别对含有和不含有干扰物质——葡萄糖和氯化钠的样品进行了测量。使用专用算法对测量数据进行了分析，得到了高相关系数 R²=0.8647。实验表明，所描述的技术能够以选择性、无标记和简单的方式检测各种 CsA 浓度水平。该方法可用于医学、兽医学和实验室诊断。